Activity-based protein profiling

by Cravatt, Benjamin F. Hsu, Ku-Lung Weerapana, Eranthie

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Title Statements

Full title :Activity-based protein profiling / editors, Benjamin F. Cravatt, Ku-Lung Hsu and Eranthie Weerapana.

Summary

This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.

Edition Statements

Publication Date:2019

Publication Statements

Publisher(s):Springer
Place of Publication:Cham, Switzerland
Language:eng
Publication Date:2019

The Physical Object

Description:1 online resource (x, 417 pages) : : illustrations (black and white, and color)

Edition Identifiers

ISBN:9783030111434, 3030111431, 9783030111441, 303011144X
DOI:10.1007/978-3-030-11143-4
oclc:on1083522325
LC Classification:QP551
Dewey Decimal:572/.6

Intro; Preface; Contents; 128 Activity-Based Protein Profiling-Enabling Multimodal Functional Studies of Microbial Communities; Abstract; 1 Introduction; 2 Probe Design; 2.1 Activity- and Affinity-Based Probes for Multimodal Characterization of Microbiome Function; 2.2 Click Chemistry and Sample Analysis; 3 Challenges of Microbiome Proteomics and How ABPP Can Facilitate; 4 Biological Applications for ABPP; 4.1 Host-Associated Microbial Communities; 4.1.1 Xenobiotic Metabolism; 4.1.2 Bile Acid Metabolism and Signaling; 4.2 Environmental Microbial Communities

4.2.1 Metabolic Activity and Translation4.2.2 Vitamin Metabolism; 4.2.3 Community Signaling; 5 Current Challenges and Outlook; References; 135 Activity-Based Protein Profiling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles; Abstract; 1 Introduction; 1.1 Activity-Based Probes: What They Are and How They Work; 2 Probe Design Strategies; 2.1 Reactive Warhead and Recognition Elements; 2.2 Linker; 2.3 Reporter; 2.3.1 Quenched Activity-Based Probes; 2.3.2 Bioorthogonal Ligation Reactions; 3 Detection Methods; 3.1 Gel-Based Detection; 3.2 Live-Cell Imaging

3.3 Mass Spectrometry4 ABPP in Bacteria; 4.1 Serine-Rea…

Subjects

Immunology Medical Microbiology protein Protéines Protéines -- Analyse Proteins Proteins -- Analysis Proteomics Protéomique SCIENCE -- Life Sciences Virology