Advances in enzyme catalysis and technologies

Front Cover -- Biomass, Biofuels, Biochemicals -- Copyright Page -- Contents -- List of contributors -- Preface -- 1 Fundamental aspects of enzymes -- 1 An introduction to enzyme structure dynamics and enzyme catalysis -- 1.1 Introduction -- 1.2 Why do some proteins work as enzyme? -- 1.2.1 Role of the active site in enzyme functioning -- 1.2.2 Cofactor, a necessity of enzyme -- 1.3 What is enzyme catalysis? -- 1.3.1 Basics of enzyme catalysis -- 1.3.2 Historical overview of enzyme catalysis theory -- 1.3.3 Mechanistic view of enzyme catalysis -- 1.4 Structural dynamics of enzymes.

1.5 Ribozymes as a nonprotein catalyst -- 1.6 Conclusions and perspective -- References -- 2 Classification of enzymes and catalytic properties -- 2.1 Introduction -- 2.1.1 Enzyme nomenclature and classification -- 2.1.2 Enzyme databases -- 2.1.3 Catalytic properties -- 2.2 Enzymes classes and properties -- 2.2.1 Oxidoreductases -- 2.2.2 Transferases -- 2.2.3 Hydrolases -- 2.2.3.1 Amylases -- 2.2.3.2 Cellulases -- 2.2.3.3 Xylanases -- 2.2.3.4 Lipases -- 2.2.3.5 Proteases -- 2.2.4 Lyases -- 2.2.5 Isomerases -- 2.2.6 Ligases -- 2.2.7 Translocases -- 2.3 Conclusions and perspectives -- References.

Further reading -- 3 Enzymes and their production strategies -- 3.1 Introduction -- 3.2 Enzymes and their classifications -- 3.3 Enzyme production -- 3.3.1 Microbial enzyme production -- 3.3.1.1 Fermentation -- 3.3.1.2 Recovery -- 3.3.1.3 Purification -- 3.3.1.4 Formulation -- 3.3.2 Enzyme production from plants and animals -- 3.4 Applications -- 3.5 Conclusions and perspectives -- Acknowledgments -- References -- 4 Robust enzymes designing for efficient biocatalysis -- 4.1 Introduction -- 4.2 Biocatalysis engineering-a driving force -- 4.3 Recent advances in protein engineering.

4.3.1 Enzyme immobilization-a drive toward optimum performance -- 4.3.2 Substrate engineering-a toolkit to harness the enzymatic promiscuity -- 4.3.3 Structure-assisted protein engineering -- 4.3.4 Advanced computational modeling -- 4.3.5 Protein engineering via directed evolution and rational design -- 4.4 Conclusions and perspectives -- 4.5 Acknowledgments -- 4.6 Conflict of interest -- References -- 2 Enzyme engineering for efficient biocatalytic reactions -- 5 Enzyme engineering strategies to confer thermostability -- 5.1 Introduction.

5.2 Enzyme engineering strategies for thermostabilization -- 5.2.1 Directed evolution -- 5.2.2 Rational/semi-rational design -- 5.2.2.1 Sequence-based engineering -- 5.2.2.1.1 Comparing sequences with higher thermostability -- 5.2.2.1.2 Consensus analysis -- 5.2.2.1.3 Ancestral sequence reconstruction -- 5.2.2.2 Structure-based engineering -- 5.2.2.2.1 Beta-factor analysis -- 5.2.2.2.2 Molecular dynamic simulations -- 5.2.2.2.3 FoldX and Rosetta_ddg -- 5.2.2.2.4 Structure-guided sequence-based engineering -- 5.2.3 De novo design -- 5.2.4 Comprehensive computational approaches.

Includes index.