Antiviral Methods and Protocols

by Gong, Edwin Yunhao

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Title Statements

Full title :Antiviral Methods and Protocols / edited by Edwin Yunhao Gong.

Summary

Although antiviral drugs have been successfully developed for some viral diseases, there remains a clear, unmet medical need to develop novel antiviral agents for the control and management of many viruses that currently have no or limited treatment options as well as a need to overcome the limitations associated with the existing antiviral drugs, such as adverse effects and emergence of drug-resistant mutations. The second edition of Antiviral Methods and Protocols features: All chapters are new and written by experts in the field, reflecting the major recent technical advances in antiviral research and discovery. This edition focuses on many important human viruses, such as human immunodeficiency virus type 1 (HIV-1), hepatitis viruses (hepatitis B and C viruses), herpes viruses, human respiratory syncytial virus (RSV), and influenza virus, while also featuring some important emerging viruses, such as dengue virus, West Nile virus, and chikungunya virus. As a volume in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Antiviral Methods and Protocols, Second Edition will serve as an excellent laboratory reference for pharmaceutical and academic biologists, medicinal chemists, and pharmacologists as well as for virologists in the field of antiviral research and drug discovery.

Edition Statements

Publication Date:2013.
Edition:2nd ed. 2013

Publication Statements

Publisher(s):Humana Press, Imprint
Place of Publication:Totowa, NJ
Language:eng
Publication Date:2013.

The Physical Object

Description:1 online resource (XIV, 452 pages 52 illustrations, 28 illustrations in color) : : online resource

Edition Identifiers

ISBN:1627034838, 9781627034838, 9781627034845, 1627034846
DOI:10.1007/978-1-62703-484-5
oclc:on1072011295
LC Classification:RM1-950
Dewey Decimal:615

A Fluorescence-Based High Throughput Screening Assay to Identify HIV-1 Inhibitors -- A Homogeneous Time-Resolved Fluorescence Assay to Identify Inhibitors of HIV-1 Fusion -- Identification of HIV-1 Reverse Transcriptase Inhibitors Using a Scintillation Proximity Assay -- Biochemical Screening Assays to Identify HIV-1 Integrase Inhibitors -- HIV-1 Genotyping of the Protease-Reverse Transcriptase and Integrase Genes to Detect Mutations that Confer Anti-Retroviral Resistance -- In Vitro Kinetic Profiling of Hepatitis C Virus NS3 Protease Inhibitors by Progress Curve Analysis -- A Novel Hepatitis C Virus NS5B Polymerase Assayof De Novo Initiated RNA Synthesis Directed from a Heteropolymeric RNA Template -- Selecting and Characterizing Drug-Resistant Hepatitis C Virus Replicon -- A Cellular Replicon-Based Phenotyping Assay to Determine Susceptibility of Hepatitis C Virus Clinical Isolates to NS3/4A Protease Inhibitors -- Expression and Purification of Hepatitis C Virus Protease from Clinical Samples -- Surface Plasmon Resonance as a Tool to Select Potent Drug Candidates for Hepatitis C Virus NS5B Polymerase -- Amplification and Sequencing of the Hepatitis C Virus NS3/4A Protease and th…