Autophagy in Stem Cell Maintenance and Differentiation

by Shravage, Bhupendra V.

Type Book

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Title Statements

Full title :Autophagy in Stem Cell Maintenance and Differentiation.

Edition Statements

Publication Date:©2023.

Publication Statements

Publisher(s):Springer International Publishing AG
Place of Publication:Cham
Language:eng
Publication Date:©2023.

The Physical Object

Description:1 online resource (290 pages).

Edition Identifiers

ISBN:9783031173622, 3031173627
oclc:on1356578807
LC Classification:RC261-271

Intro -- Preface -- Contents -- 1 Assays for Monitoring Autophagy in Stem Cells -- Introduction -- Autophagy Process and Mechanism -- Techniques for Assessing Autophagy Orchestration -- Electron Microscopy Imaging -- Fluorescence Microscopy -- LC3 Antibody Tagging -- Fluorescent Protein Tagging of LC3 -- mRFP/mCherry-GFP-LC3 Tandem Reporter Constructs and Their Advancements -- GFP-LC3-RFP-LC3∆G -- SQSTM/p62/Ref(2)P Tagging -- Evaluation of Specific Steps During Autophagy -- Autophagy Visualization Dyes -- Tracking Mitophagy in Stem Cells -- Atg8s/LC3 and p62 Protein Expression in Stem Cells -- LC3 Turnover Assays -- Flow Cytometry and Fluorescence-Associated Cell Sorting (FACS) -- Potential Probes for Monitoring Autophagy -- Gene Expression Assays -- Protein Interaction Databases -- Conclusion -- References -- 2 Autophagy in Stem Cell Maintenance and Differentiation -- Introduction -- Autophagy Compared to Necrosis and Apoptosis -- The Autophagic Cascade -- Types of Autophagy -- Role of Autophagy in Stem Cell Maintenance -- Role of Autophagy in Maintenance of Hematopoietic Stem Cells (HSCs) -- Role of Autophagy in Maintenance of Neural Stem Cells -- Role of Autophagy in Maintenanc…