Bioanalytical chemistry

Interdisciplinary knowledge is becoming increasingly important to the modem scientist. This invaluable textbook covers bioanalytical chemistry (mainly the analysis of proteins and DNA) and explains everything for the non-biologist. Electrophoresis, mass spectrometry, biosensors, bioassays, DNA and protein sequencing are not necessarily all included in conventional analytical chemistry textbooks. The book describes the basic principles and the applications of instrumental and molecular methods. It is particularly useful to chemistry and engineering students who already have some basic knowledge about analytical chemistry. This revised second edition contains a new chapter on optical spectroscopy, and updated methods and new references throughout.

Previous edition: Andreas Manz. Bioanalytical chemistry (Imperial College Press, 2004).

1.1.Amino Acids, Peptides and Proteins -- 1.1.1.Amino Acids -- 1.1.2.Peptides and Proteins -- 1.2.Nucleic Acids -- 1.2.1.The Structure of Nucleic Acids -- 1.2.2.Synthesis of Proteins -- 1.3.Biomolecules in Analytical Chemistry -- 1.3.1.Classical Analytical Chemistry -- 1.3.2.Limitations of Classical Analytical Chemistry -- 1.3.3.Bioanalytical Chemistry -- Summary -- References -- 2.1.The Principle of Chromatography -- 2.2.Basic Chromatographic Theory -- 2.3.Liquid Chromatography Techniques for Bioanalysis -- 2.3.1.Liquid Chromatography Instrumentation -- 2.3.2.Reversed-Phase Liquid Chromatography (RP-LC) -- 2.3.3.Hydrophilic Interaction Liquid Chromatography (HILIC) -- 2.3.4.Ion-Exchange Chromatography -- 2.3.5.Affinity Chromatography -- 2.3.6.Size Exclusion Chromatography (SEC) -- Summary -- References -- 3.1.Principle and Theory of Electrophoresis -- 3.1.1.Electrophoretic Mobility -- 3.1.2.Joule Heating -- 3.1.3.Electroosmotic Flow (EOF) -- 3.1.4.Separation Efficiency and Resolution -- 3.2.Gel Electrophoresis (GE) -- 3.2.1.Instrumentation for Gel Electrophoresis -- 3.2.2.Modes of Gel Electrophoresis -- 3.2.3.Sodium-Dodecyl Sulfate [--] Polyacryl Amide Gel Electrophoresis (SDS-PA…