Biointerface characterization by advanced IR spectroscopy

IR spectroscopy has become without any doubt a key technique to answer questions raised when studying the interaction of proteins or peptides with solid surfaces for a fundamental point of view as well as for technological applications. Principle, experimental set ups, parameters and interpretation rules of several advanced IR-based techniques; application to biointerface characterisation through the presentation of recent examples, will be given in this book. It will describe how to characterise amino acids, protein or bacterial strain interactions with metal and oxide surfaces, by using infrared spectroscopy, in vacuum, in the air or in an aqueous medium. Results will highlight the performances and perspectives of the technique. Description of the principles, expermental setups and parameter interpretation, and the theory for several advanced IR-based techniques for interface characterisation. Contains examples which demonstrate the capacity, potential and limits of the IR techniques. Helps finding the most adequate mode of analysis. Contains examples. Contains a glossary by techniques and by keywords.

Cover; Biointerface Characterization by Advanced IR Spectroscopy; Copyright; Contents; Preface; Contributers; Chapter -- 1 RAIRS under ultrahigh vacuum conditions on metal surfaces; Chapter -- 2 PM-IRRAS at liquid interfaces; Chapter -- 3 Infrared spectroscopy for characterization of biomolecular interfaces; Chapter -- 4 Infrared analysis of biomolecule attachment to functionalized silicon surfaces; Chapter -- 5 Attenuated total reflection infrared (ATR-IR) spectroscopy, modulation excitation spectroscopy (MES), and vibrational circular dichroism (VCD)

Chapter -- 6 Synchrotron infrared interface scienceChapter -- 7 IR spectroscopy for biorecognition and molecular sensing; Chapter -- 8 Advanced infrared glasses forbiochemical sensing; Chapter -- 9 AFM-IR: photothermal infrared nanospectroscopy; Chapter -- 10 Sum-frequency generation spectroscopy of biointerfaces; Index