Title Statements
- Full title :Biological microtechnique / J.B. Sanderson.
Summary
A completely new practical guide to both new and classical methods of slide-making which is easy-to-read and easy-to-understand. Biological Microtechnique contains a wealth of practical detail which will provide a firm grounding in preparative methods for light microscopy.
Read More ↓Edition Statements
- Publication Date:1994.
Publication Statements
- Publisher(s):BIOS Scientific Publishers in association with the Royal Microscopical Society
- Place of Publication:Oxford
- Language:eng
- Publication Date:1994.
The Physical Object
- Description:1 online resource.
Edition Identifiers
- ISBN:9781000102413, 1000102416, 9781003077008, 1003077005, 9781000119619, 1000119610, 9781000140941, 1000140946
- oclc:on1178640682
- LC Classification:QH207.S25 1994
- Dewey Decimal:578
Table of Contents
Cover -- Half Title -- Title Page -- Copyright Page -- Preface -- Acknowledgements -- Table of contents -- Abbreviations -- Chapter 1: Introduction -- Collecting material for specimen preparation -- Choice of preparative technique -- Looking at preparations -- References -- Chapter 2: Fixation -- Function and use of fixatives -- Methods of fixation -- Immersion fixation -- Perfusion fixation -- Vapour fixation -- Phase-partition fixation -- Mechanical methods -- When not to use fixation -- The penetration of fixatives -- Osmolarity and pH -- Fixing agents -- Formaldehyde
Glutaraldehyde and acrolein -- Alcohols and acetone -- Mercuric chloride -- Potassium dichromate -- Picric acid and acetic acid -- Osmium tetroxide -- Fixative mixtures -- Secondary fixation -- Preservatives -- Washing tissues -- Microwaves in histology -- Microwaving formalin-fixed tissues -- Leiden fixative -- Microwaving paraffin sections -- Microwaving cryostat sections -- Staining reactions -- Safety -- References -- Chapter 3: Tissue Processing -- Dehydration -- Transition media -- Processing schedules -- Automatic processing -- Embedding media -- Wax embedding -- Preparation for cutting
Ribboning -- Ribbo…
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