Biological microtechnique

Biological microtechnique

by Sanderson, J. B., (Jeremy B.)

Type Book
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A completely new practical guide to both new and classical methods of slide-making which is easy-to-read and easy-to-understand. Biological Microtechnique contains a wealth of practical detail which will provide a firm grounding in preparative methods for light microscopy.

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Title Statements

  • Full title :Biological microtechnique / J.B. Sanderson.

Summary

A completely new practical guide to both new and classical methods of slide-making which is easy-to-read and easy-to-understand. Biological Microtechnique contains a wealth of practical detail which will provide a firm grounding in preparative methods for light microscopy.

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Edition Statements

  • Publication Date:1994.

Publication Statements

The Physical Object

  • Description:1 online resource.

Edition Identifiers

  • ISBN:9781000102413, 1000102416, 9781003077008, 1003077005, 9781000119619, 1000119610, 9781000140941, 1000140946
  • oclc:on1178640682
  • LC Classification:QH207.S25 1994
  • Dewey Decimal:578

Table of Contents

Cover -- Half Title -- Title Page -- Copyright Page -- Preface -- Acknowledgements -- Table of contents -- Abbreviations -- Chapter 1: Introduction -- Collecting material for specimen preparation -- Choice of preparative technique -- Looking at preparations -- References -- Chapter 2: Fixation -- Function and use of fixatives -- Methods of fixation -- Immersion fixation -- Perfusion fixation -- Vapour fixation -- Phase-partition fixation -- Mechanical methods -- When not to use fixation -- The penetration of fixatives -- Osmolarity and pH -- Fixing agents -- Formaldehyde

Glutaraldehyde and acrolein -- Alcohols and acetone -- Mercuric chloride -- Potassium dichromate -- Picric acid and acetic acid -- Osmium tetroxide -- Fixative mixtures -- Secondary fixation -- Preservatives -- Washing tissues -- Microwaves in histology -- Microwaving formalin-fixed tissues -- Leiden fixative -- Microwaving paraffin sections -- Microwaving cryostat sections -- Staining reactions -- Safety -- References -- Chapter 3: Tissue Processing -- Dehydration -- Transition media -- Processing schedules -- Automatic processing -- Embedding media -- Wax embedding -- Preparation for cutting

Ribboning -- Ribbo…

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