Bioluminescence: methods and protocols

by Kim, Sung-Bae, (Writer on bioluminescence)

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Title Statements

Full title :Bioluminescence : methods and protocols. / edited by Sung-Bae Kim.

Summary

This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 1 delves into techniques for the establishment of luciferins and luciferases, basic in vitro and in vivo applications, as well as protocols on multiplex imaging platforms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 1 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.

Edition Statements

Publication Date:2022.
Edition:Fourth edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2022.

Notes

Includes index.

The Physical Object

Description:1 online resource (xvi, 474 pages) : : illustrations (some color).

Edition Identifiers

ISBN:9781071624531, 1071624539
DOI:10.1007/978-1-0716-2453-1
oclc:on1336709615
LC Classification:QH641
Dewey Decimal:572/.4358

Gene Cloning and Functional Analysis of the Luciferase from Luminous Syllids of the Genus Odontosyllis -- Synthetic Coelenterazine Derivatives and Their Application for Bioluminescence Imaging -- Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging -- Biosynthesis-Inspired Deracemizative Production of D-Luciferin In Vitro by Combining Luciferase and Thioesterase -- Production of Metridia Luciferase in Native Form by Oxidative Refolding from E. coli Inclusion Bodies -- Production of Copepod Luciferases via Baculovirus Expression System -- Molecular Tension Probe for In Vitro Bioassays -- Optimized Loop-Mediated Amplification (LAMP) Allows Single Copy Detection Using Bioluminescent Assay in Real Time (BART) -- A Simple and Rapid Bioluminescence-Based Functional Assay of Organic Anion Transporter 1 as a d-Luciferin Transporter -- A Simple Bioluminescent Assay for the Screening of Cytotoxic Molecules against the Intracellular Form of Leishmania infantum -- A Simple, Robust, and Affordable Bioluminescent Assay for Drug Screening against Infective African Trypanosomes -- Imaging of Autonomous Bioluminescence Emission from Single Mammalian Cells -- Rapid Single-Cell Det…