Building a cell from its component parts

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This new volume of Methods in Cell Biology looks at building a cell from its component parts. Chapters cover such topics as engineering motor scaffolds, artificial cytoskeletons, interconnected droplet networks and artificial cells; building cytoskeletal systems and artificial actin cortex on pillar arrays; reconstituting membrane fission, actin bundles at membranes, actin cortex in droplets, dynein-dynactin mediated cargo transport, MinD systems, protein-lipid machines that transform membranes…

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Title Statements

Full title :Building a cell from its component parts / edited by Jennifer Ross, Wallace F. Marshall.

Summary

Edition Statements

Publication Date:2015.
Edition:First edition

Publication Statements

Publisher(s):Academic Press
Place of Publication:Amsterdam
Language:eng
Publication Date:2015.

The Physical Object

Description:1 online resource : : color illustrations

Edition Identifiers

ISBN:9780128026397, 0128026391, 012802450X, 9780128024508
oclc:ocn909368064
LC Classification:QH585.2
Dewey Decimal:571.638

Front Cover; Methods in Cell Biology; Series Editors; Methods in Cell Biology; Copyright; Contents; Contributors; Preface; 1. In vitro systems for the study of microtubule-based cell polarity in fission yeast; Introduction; 1. Rationale; 2. Materials; 2.1 Materials for Method 1: Elongated Glass Wells with TiO2 Overhang; 2.1.1 Microfabrication; 2.1.2 Surface functionalization; 2.1.3 Assay; 2.2 Materials for Method 2: Elongated Water in Oil Emulsion Droplets; 2.2.1 Microfluidic chip; 3. Methods; 3.1 Method 1: Elongated Glass Wells with TiO2 Overhang; 3.1.1 Design.

3.1.2 Deposition of chromium and titanium oxide by electron-beam evaporation3.1.3 Postannealing of titanium oxide; 3.1.4 UV-lithography with S1813; 3.1.5 Reactive ion etching; 3.1.6 Wet etching with KOH; 3.1.7 Surface functionalization; 3.1.8 Assays; 3.2 Method 2: Elongated Water in Oil Emulsion Droplets; 3.2.1 Microfluidic design; 3.2.2 Masks for microfluidic chip fabrication; 3.2.3 SU-8 negative mould; 3.2.4 PDMS chip (Figure 5); 3.2.5 Microfluidic setup and droplet formation; 3.2.6 Lipids composition; 3.2.7 Assays; Discussion and Perspectives; Acknowledgments; References.

2. Microtubules, MAPs, and motor patternsIntro…