Cardiac gene therapy: methods and protocols
This second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary mate…
Read More ↓Title Statements
- Full title :Cardiac gene therapy : methods and protocols / edited by Kiyotake Ishikawa.
Summary
This second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cardiac Gene Therapy: Methods and Protocols, Second Edition is a valuable tool for researchers in cardiology who are conducting gene therapy research. This book will lead to further advancements and successful clinical translations in the field. .
Read More ↓Edition Statements
- Publication Date:©2022
- Edition:Second edition
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York, NY
- Language:eng
- Publication Date:©2022
Notes
Includes index.
The Physical Object
- Description:1 online resource (xiv, 347 pages) : : illustrations (chiefly color).
Edition Identifiers
- ISBN:9781071627075, 1071627074
- DOI:10.1007/978-1-0716-2707-5
- oclc:on1343196860
- LC Classification:RC669
- Dewey Decimal:616.1/042
Table of Contents
Updates on Cardiac Gene Therapy Research and Methods: Overview of Cardiac Gene Therapy -- Tough Decoy-Mediated Cardiac Gene Suppression -- Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes using miRcombo -- CRISPR/Cas9 Gene Editing of RYR2 in Human iPSC Derived Cardiomyocytes to Probe Ca2+ Signaling Aberrancies of CPVT Arrhythmogenesis -- Enhancing Cardiomyocyte Transcription using In Vivo CRISPR/Cas9 Systems -- AAV Mediated Somatic Gene Editing for Cardiac and Skeletal Muscle in a Large Animal Model -- Optimization of Synthesis of Modified mRNA -- Design and Production of Heart Chamber-Specific AAV9 Vectors -- Generation of Atrial-Specific Construct using Sarcolipin Promoter Associated CRM4 Enhancer -- Cardiac Targeted Adeno-Associated Virus Injection in Rats -- Cardiac Gene Delivery in Large Animal Models-Antegrade Techniques -- Locked Nucleic Acid AntimiR Therapy for the Heart -- Cardiac Gene Delivery in Large Animal Models: Selective Retrograde Venous Injection -- Endocardial Gene Delivery using NOGA Catheter System -- Surgical Methods for Cardiac Gene Delivery in Large Animals -- Atrial Gene Painting in Large Animal Model of Atrial Fibrillati…
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