Cell cycle synchronization: methods and protocols

by Banfalvi, Gaspar

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Title Statements

Full title :Cell cycle synchronization : methods and protocols / edited by Gaspar Banfalvi.

Summary

[Publisher-supplied data] To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the arrest-and-release approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.

Edition Statements

Publication Date:©2011.

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:©2011.

The Physical Object

Description:xii, 295 pages : : illustrations ; : 27 cm

Edition Identifiers

ISBN:1617791814, 9781617791819, 9781617791826, 1617791822
oclc:ocn786244851
LC Classification:QH605
Dewey Decimal:571.84

1. Overview of cell synchronization / Gaspar Banfalvi -- 2. Synchronization of mammalian cells and nuclei by centrifugal elutriatio / Gaspar Banfalvi -- 3. Cytofluorometric purification of diploid and tetraploid cancer cells / Maria Castedo, Lorenzo Galluzzi, Ilio Vitale, Laura Senovilla, Didier Métivier, Mohamed Jèmaà, Santiago Rello-Varona, and Guido Kroemer -- 4. Large-scale mitotic cell synchronization / Kalyan Dulla and Anna Santamaria -- 5. Synchronization of mammalian cell cultures by serum deprivation / Thomas J. Langan and Richard C. Chou -- 6. Cell synchronization by inhibitors of DNA replication induces replication stress and DNA damage response: analysis by flow cytometry / Zbigniew Darzynkiewicz, H. Dorota Halicka, Hong Zhao, and Monika Podhorecka -- 7. Chromosome formation during fertilization in eggs of the teleost oryzias latipes / Takashi Iwamatsu -- 8. Specific cell cycle synchronization with butyrate and cell cycle analysis / Congjun Li -- 9. Analysis of nuclear uracil-DNA glycosylase (nUDG) turnover during the cell cycle / Jennifer A. Fischer and Salvatore Caradonna -- 10. Synchronization of HeLa cells / Hoi Tang Ma and Randy Y.C. Poon -- 11. Synchronization of…

Subjects

Cell culture Cell culture -- Laboratory manuals Cell Culture Techniques -- methods Cell Cycle Cell cycle -- Laboratory manuals. -- Re… Cell cycle -- Regulation Cellules -- Manuels de laboratoire. -- … Cycle cellulaire Cycle cellulaire -- Manuels de laborato… Laboratory Manual Laboratory manuals Manuels de laboratoire Zellkultur Zellwachstum Zellzyklus