Centrosome & centriole

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This new volume of Methods in Cell Biology looks at methods for analyzing centrosomes and centrioles. Chapters cover such topics as methods to analyze centrosomes, centriole biogenesis and function in multi-ciliated cells, laser manipulation of centrosomes or CLEM, analysis of centrosomes in human cancers and tissues, proximity interaction techniques to study centrosomes, and genome engineering for creating conditional alleles in human cells.

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Title Statements

Full title :Centrosome & centriole / [edited by] Renata Basto and Karen Oegema.

Summary

Edition Statements

Publication Date:©2015

Publication Statements

Publisher(s):Academic Press
Place of Publication:Amsterdam
Language:eng
Publication Date:©2015

The Physical Object

Description:1 online resource (xix, 414 pages) : : illustrations (some color)

Edition Identifiers

ISBN:9780128026380, 0128026383
oclc:ocn913876361
LC Classification:QH597
Dewey Decimal:571.6/5

1. Correlative Light and Electron Microscopy Analysis of the Centrosome; A Step by Step Protocol. Dong Kong and Jadranka Loncarek 2.Generation of a Conditional Analog-Sensitive Kinase in Human Cells Using CRISPR/Cas9-Mediated Genome Engineering Tyler Moyer and Andrew J. Holland 3. Studying Centrosome Function Using Three-Dimensional Cell Cultures Teresa Arnandis and Susana A. Godinho 4. Analysis of Centrosomes in Human Cancer Stefan Duensing 5. In Vivo Examination of the Cortical Cytoskeleton in Multiciliated Cells Using Electron Tomography Daniel K. Clare, Maud Dumoux and Delphine Delacour 6.Tackling Centrosome Biology Through Gene Targeting in Chicken B Cells Pavithra L. Chavali and Fanni Gergely 7. Centriole Biogenesis and Function in Multi-Ciliated Cells Siwei Zhang and Brian J. Mitchell 8. Sub-Diffraction Resolution Microscopy Methods for Analyzing Centrosomes Organization Vito Mennella, Rachel Hanna and Moshe Kim 9. Probing Mammalian Centrosome Structure Using Bioid Proximity-Dependent Biotinylation Elif Nur Firat-Karalar and Tim Stearns 10. Purification of Centrosomes from Mammalian Cell Lines Delphine Gogendeau, Paul…