Chloroplast biotechnology: methods and protocols
by Maliga, P.
In Chloroplast Biotechnology: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used in chloroplast molecular biology. Chapters focus on essential background information, applications in tobacco, and protocols for plastid transformation in crops, and Chlamydomonas and Bryophytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary mate…
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- Full title :Chloroplast biotechnology : methods and protocols / edited by Pal Maliga.
Summary
In Chloroplast Biotechnology: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used in chloroplast molecular biology. Chapters focus on essential background information, applications in tobacco, and protocols for plastid transformation in crops, and Chlamydomonas and Bryophytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoidance of known pitfalls. Authoritative and practical, Chloroplast Biotechnology: Methods and Protocols seeks to aid scientists who study chloroplast molecular biology as well as those interested in applications in agriculture, industrial biotechnology, and healthcare.--
Read More ↓Edition Statements
- Publication Date:2014
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York
- Language:eng
- Publication Date:2014
The Physical Object
- Description:xv, 452 pages : : illustrations ; : 27 cm
Edition Identifiers
- ISBN:9781627039949, 1627039945, 1627039953, 9781627039956
- oclc:ocn870978500
- LC Classification:QK725
- Dewey Decimal:571.6592
Table of Contents
The Plastid Genomes of Flowering Plants -- Next Generation Technologies to Determine Plastid Genome Sequences -- Plastid Gene Transcription: Promoters and RNA Polymerases -- Plastid mRNA Translation -- Engineering Chloroplasts for High-Level Foreign Protein Expression -- Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences -- Fluorescent Labeling and Confocal Microscopic Imaging of Chloroplasts and Non-Green Plastids -- Plastid Transformation in Nicotiana tabacum and Nicotiana sylvestris by Biolistic DNA Delivery to Leaves -- Nicotiana tabacum PEG-mediated Plastid Transformation -- Plastid Transformation of Tobacco Suspension Cell Cultures -- Tryptophan and Indole Analog Mediated Plastid Transformation -- Plastid Marker Gene Excision in Greenhouse-Grown Tobacco by Agrobacterium-Delivered Cre Recombinase -- Determination of the Half-Life of Chloroplast Transcripts in Tobacco Leaves -- Quantification of Organellar DNA and RNA Using Real-Time PCR -- Plastid Transformation for Rubisco Engineering and Protocols for Assessing Expression -- Plastid Transformation in Tomato -- Stable Plastid Transformation of Petunia -- Plastid Transformation in Potato -- Solanum tuberos…
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