Chromatography: Basic Principles, Sample Preparations and Related Methods

Finally a book on chromatography which is easy to grasp for undergraduates and technicians; covers the area in sufficient depth while still being concise. The book includes all recent technology advances and has core textbook features further improving the learning experience. This book is the perfect introduction into a methodology which is the underlying principle of the vast majority of separation methods worldwide. Everyone working in a lab environment must be familiar with the basis of these technologies and Tyge Greibrokk, Elsa Lundanes and Leon Reubsaet succeed in delivering a text which is easy to read for undergraduates and laboratory technicians, and covers the area in sufficient depth while still being concise. The book includes all recent technology advances and has core textbook features further improving the learning experience. Importantly, the text does not only cover all major modern chromatography technology (thin layer, gas, high pressure liquid, and supercritical fluid chromatography) but also related methods, in particular electrophoretic technologies.

9.6.1.6 How to Analyze the Dialysate?.

Chromatography: Basic Principles, Sample Preparations and Related Methods -- Contents -- Preface -- 1 General Concepts -- 1.1 Introduction -- 1.2 Migration and Retention -- 1.2.1 General -- 1.2.2 Mobile and Stationary Phases -- 1.2.3 Chromatograms -- 1.2.4 Retention Factor -- 1.3 Band Broadening -- 1.3.1 Eddy Diffusion -- 1.3.2 Longitudinal Diffusion -- 1.3.3 Resistance to Mass Transfer -- 1.3.4 Combined Band Broadening in a Column -- 1.3.5 Band Broadening outside the Column -- 1.4 Measuring Column Efficiency -- 1.4.1 Plate Numbers -- 1.4.2 Coupling Columns -- 1.4.3 Plate Height -- 1.4.3.1 Reduced Plate Height -- 1.4.4 Effective Plate Number -- 1.4.5 Asymmetry -- 1.5 Resolution -- 1.5.1 Increasing the Resolution -- 1.6 Peak Capacity -- 1.7 Two-Dimensional Systems -- 1.8 Increased Performance -- References -- 2 Gas Chromatography -- 2.1 Introduction -- 2.2 Mobile Phase/Carrier Gas -- 2.3 Injection Systems -- 2.3.1 Packed Column Injector (Evaporation Injector) -- 2.3.2 Injection Systems for Capillary Columns -- 2.3.2.1 Split Injection -- 2.3.2.2 Splitless Injection -- 2.3.2.3 On-Column Injection -- 2.3.2.4 Large-Volume Injectors -- 2.3.2.5 Headspace Techniques -- 2.4 Columns -- 2.4.…