Deubiquitinases

Deubiquitinases: methods and protocols

by Edelmann, Mariola J. Maupin-Furlow, Julie

Type Book
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This book aims to advance the understanding of deubiquitinases (DUBs) and DUB-like enzymes. Chapters detail methods used to identify, classify,and biochemically characterize DUBs along with approaches that enable both the determination and alteration of DUB biological function.. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known …

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Title Statements

  • Full title :Deubiquitinases : methods and protocols / edited by Julie Maupin-Furlow, Mariola J. Edelmann.

Summary

This book aims to advance the understanding of deubiquitinases (DUBs) and DUB-like enzymes. Chapters detail methods used to identify, classify,and biochemically characterize DUBs along with approaches that enable both the determination and alteration of DUB biological function.. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Deubiquitinases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .

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Edition Statements

  • Publication Date:©2023

Publication Statements

  • Publisher(s):Humana Press
  • Place of Publication:New York, NY
  • Language:eng
  • Publication Date:©2023

Notes

Includes index.

The Physical Object

  • Description:1 online resource (xiii, 300 pages) : : illustrations (chiefly color).

Edition Identifiers

  • ISBN:9781071628034, 1071628038
  • DOI:10.1007/978-1-0716-2803-4
  • oclc:on1350841884
  • LC Classification:QP552.U24
  • Dewey Decimal:612/.01575

Table of Contents

Characterization of Deubiquitinase Catalytic State Using a Structure-Based Approach -- Synthetic Diubiquitin Fluorogenic Substrates to Study DUBs -- Fluorometric Characterization of DUB Activity: From Single Enzyme Reactions to Live Intact Cell -- Using Activity-Based Proteomics for the Quantification of Deubiquitinases in Animal Tissue -- Design and Synthesis of Ubiquitin-Based Chemical tools with Unnatural Amino Acids for Selective Detection of Deubiquitinases -- High Throughput Assay for Characterizing Rpn11 Deubiquitinase Activity -- Activity-Based Protein Profiling (ABPP) for Cellular Deubiquitinase (DUB) and Inhibitor Profiling at Deep and High-Throughput Levels -- High-Throughput MALDI-TOF Mass-Spectrometry-based Deubiquitylating Enzyme Assay for Drug Discovery -- Bioinformatical Approaches to The Discovery and Classification of Novel Deubiquitinases -- Application of Archaea in Deubiquitinase-Like Enzyme Discovery and Activity Assay -- Characterization of the Ubiquitin and ISG15 Deconjugase Activity of SARS-CoV-1 and 2 Papain-like Protease -- Next-generation Phage Display to Identify Peptide Ligands of Deubiquitinases -- Identification of Deubiquitinase Substrates in Xenop…

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Subjects

Ubiquitin

Writers

Edelmann, Mariola J. Maupin-Furlow, Julie

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