Directed evolution: methods and protocols
by Currin, Andrew Swainston, Neil
This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the high…
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- Full title :Directed evolution : methods and protocols / edited by Andrew Currin and Neil Swainston.
Summary
This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.
Read More ↓Edition Statements
- Publication Date:2022
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York, NY
- Language:eng
- Publication Date:2022
Notes
Formerly CIP.
The Physical Object
- Description:xi, 278 pages : : illustrations (some color) ; : 26 cm.
Edition Identifiers
- ISBN:9781071621516, 1071621513
- oclc:on1338652326
- LC Classification:QH390
- Dewey Decimal:572.8
Table of Contents
Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach -- Recombination of Single Beneficial Substitutions Obtained from Protein Engineering by Computer-Assisted Recombination (CompassR) -- Non-Degenerate Saturation Mutagenesis: Library Construction and Analysis via MAX and ProxiMAX Randomization -- Antha-Guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries -- SpeedyGenesXL An Automated, High-Throughput Platform for the Preparation of Bespoke Ultra-Large Variant Libraries for Directed Evolution -- Facile Assembly of Combinatorial Mutagenesis Libraries using Nicking Mutagenesis -- GeneORator: An Efficient Method for the Systematic Mutagenesis of Entire Genes -- Rapid Cloning of Random Mutagenesis Libraries using PTO-QuickStep -- Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs -- Application of Restriction Free (RF) Cloning in Circular Permutation -- Site-Directed Mutagenesis Method Mediated by Cas9 -- Directed Evolution of Transcription Factor-Based Biosensors for Altered Effector Specificity -- A Screening Method for P450 BM3 Mutant Libraries using Multiplexed Capillary Electrophoresis for …
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