G protein-coupled receptors: signaling, trafficking and regulation

by Shukla, Arun K.

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Title Statements

Full title :G protein-coupled receptors : signaling, trafficking and regulation / edited by Arun K. Shukla.

Edition Statements

Publication Date:2016.
Edition:First edition

Publication Statements

Publisher(s):Academic Press is an imprint of Elsevier
Place of Publication:Cambridge, MA, USA
Language:eng
Publication Date:2016.

Notes

VERSEMENT EN LOT.

The Physical Object

Description:1 ressource en ligne.

Edition Identifiers

ISBN:9780128036129, 0128036125
oclc:ocn987668556
LC Classification:QH585
Dewey Decimal:571.6

Front Cover; Series Editors; Methods in Cell Biology G Protein-Coupled Receptors: Signaling, Trafficking and Regulation; Copyright; Contents; Contributors; Preface; 01 -- Trafficking, Localization andImaging of GPCRs; 1 -- Localization and signaling of GPCRs in lipid rafts; INTRODUCTION; 1. LOCALIZATION OF GPCRS IN LIPID RAFTS; 1.1 Isolation of Lipid Rafts; 1.1.1 Detergent-free method; 1.1.2 Detergent-based method; 1.1.3 Immunoblotting and data interpretation; 1.2 Localization of GPCRs in Lipid Rafts; 1.2.1 Cells in suspension; 1.2.2 Adherent cells; 2. GPCR SIGNALING IN LIPID RAFTS.

2.1 Perturbation of Raft Stability2.2 Changing the Cholesterol Content; 2.3 Fluorescence Imaging; REFERENCES; 2 -- Imaging GPCRs trafficking and signaling with total internal reflection fluorescence microscopy in cultured neurons; 1. IMAGE ACQUISITION; 1.1 Important Considerations before Imaging; 1.2 Materials; 1.2.1 Cell culture; 1.2.2 TIRF microscopy equipment and settings; 1.3 Imaging; 1.3.1 Cell culture; 1.3.2 Live cell imaging; 1.4 Notes; 2. IMAGE ANALYSIS; 3. FINAL COMMENTS; Acknowledgments; REFERENCES; 3 -- Trafficking of ciliary G protein-coupled receptors; INTRODUCTION.

1. RHODOPSIN AS A CIL…

Subjects

Cell receptors Cytological Techniques Cytologie -- Technique Cytology -- Technique e-books G proteins GTP-Binding Proteins Livres numériques Proteïnes G. Récepteurs cellulaires Receptors, Cell Surface