Glycosyltransferases

Glycosyltransferases: methods and protocols

by Brockhausen, Inka

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Glycosyltransferases (GTs) are essential for the biosynthesis of complex glycoconjugates and are powerful tools to study the functions of complex glycans in health, development and disease. Complex glycoconjugates, such as glycoproteins, proteoglycans and glycolipids, are assembled by GTs which synthesize specific linkages between sugars or sugars and protein. This is in contrast to the non-specific or less specific chemical glycation reactions, transglycosylation and reverse glycosylation reac…

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Title Statements

  • Full title :Glycosyltransferases : methods and protocols / edited by Inka Brockhausen.

Summary

Glycosyltransferases (GTs) are essential for the biosynthesis of complex glycoconjugates and are powerful tools to study the functions of complex glycans in health, development and disease. Complex glycoconjugates, such as glycoproteins, proteoglycans and glycolipids, are assembled by GTs which synthesize specific linkages between sugars or sugars and protein. This is in contrast to the non-specific or less specific chemical glycation reactions, transglycosylation and reverse glycosylation reactions. Glycosyltransferases: Methods and Protocols contains a wide range of studies, methods and protocols which form a solid basis for investigations of the role and mechanisms, biology and pathology involving GTs. Written in the successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Glycosyltransferases: Methods and Protocols is a vital contribution to glycobiology and glycopathology, and to applications of these enzymes in biotechnology and drug development. It will prove invaluable to students, postdoctoral fellows, and senior scientists carrying on research of GTs that has been intensified over the last years

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Edition Statements

  • Publication Date:©2013.

Publication Statements

  • Publisher(s):Humana Press
  • Place of Publication:New York
  • Language:eng
  • Publication Date:©2013.

The Physical Object

  • Description:1 online resource (xvii, 425 pages) : : illustrations

Edition Identifiers

  • ISBN:9781627034654, 162703465X, 1627034641, 9781627034647
  • DOI:10.1007/978-1-62703-465-4
  • oclc:ocn850907340
  • LC Classification:QP606.G6
  • Dewey Decimal:572/.792

Table of Contents

Glycan microarray screening assay for glycosyltransferase specificities / W. Peng, C.M. Nycholat and N. Razi -- A fluorescence-based assay for core 1 [beta]3galactosyltransferase (T-synthase) activity / T. Ju and R.D. Cummings -- Structural and biochemical analysis of a bacterial glycosyltransferase / F. Zhu [and others] -- Study of the biological functions of mucin type core 3 O-glycans / S.H. Lee and M. Fukuda -- Generation of anti-sulfated glycan antibodies using sulfotransferase-deficient mice / H. Kawashima -- Fluorescent microscopy as a tool to elucidate dysfunction and mislocalization of golgi glycosyltransferases in COG complex depleted mammalian cells / R.A. Willett, I.D. Pokrovskaya and V.V. Lupashin -- A practical approach to reconstruct evolutionary history of animal sialyltransferases and gain insights into the sequence-function relationships of golgi-glycosyltransferases / D. Petit [and others] -- Fluorescent lectin staining of drosophila embryos and tissues to detect the spatial distribution of glycans during development / E. Tian, L. Zhang and K.G. Ten Hagen -- Photoaffinity labeling of protein O-mannosyltransferases of the PMT1/PMT2 subfamily / M. Loibl and S. Str…

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Subjects

Animal Biochemistry Biochemical Phenomena Biochemie biochemistry Carbohydrate Chemistry Carbohydrate Metabolism Carbohydrates Chemical Phenomena Chemicals and Drugs Chemistry, Organic Eiwitten en enzymen enzyme Enzymes Enzymes and Coenzymes food chemistry Glycosylation glycosyltransferasen Glycosyltransférases Glycosyltransferases -- Laboratory manu… Glycosyltransférases -- Manuels de labo…

Writers

Brockhausen, Inka

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