HDAC/HAT function assessment and inhibitor development: methods and protocols

by Krämer, Oliver H.

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Title Statements

Full title :HDAC/HAT function assessment and inhibitor development : methods and protocols / edited by Oliver H. Krämer.

Summary

This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors explore the generation and evaluation of novel histone deacetylase inhibitors and new and improved techniques to assess acetylation-dependent molecular mechanisms in vitro and in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to further elucidate this vital area of study.

Edition Statements

Publication Date:2022
Edition:Second edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:2022

Notes

Formerly CIP.

The Physical Object

Description:xvii, 511 pages : : illustrations (some color) ; : 26 cm.

Edition Identifiers

ISBN:9781071627877, 1071627872
oclc:on1346943412
LC Classification:QP552.H5
Dewey Decimal:572.66

Analyzing Lymphoma Development and Progression Using HDACi in Mouse Models -- Colony Formation Assay to Test the Impact of HDACi on Leukemic Cells -- Defined Human Leukemic CD34+ Liquid Cultures to Study HDAC/Transcriptional Repressor Complexes -- Development of a Cellular Model Mimicking Specific HDAC Inhibitors -- Evaluation of Anti-Tumor and On-Target Activity of HDAC Inhibitors with the Zebrafish Embryo Xenograft Model -- Human Platelet Lysate as Valid Cell Growth Additive to Assess Protein Acetylation -- Investigating Physiopathological Roles for Sirtuins in a Mouse Model -- Patient-Derived Organoids for In Vivo Validation of In Vitro Data -- Analysis of HDACi-Coupled Nanoparticles: Opportunities and Challenges -- Development of Pyrazine-Anilinobenzamides as Histone Deacetylase HDAC1-3 Selective Inhibitors and Biological Testing Against Pancreas Cancer Cell Lines -- Evaluation of Small Molecule HDAC Inhibitors through In Vitro and In Cellulo Approaches -- Synthesis, Biochemical, and Cellular Evaluation of HDAC6 Targeting Proteolysis Targeting Chimeras -- HDACi Delivery Systems Based on Cellulose Valproate Nanoparticles -- Synthesis and Characterization of Reversible Covalent …