Hepatitis C methods and protocols

by Tang, Hengli

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Title Statements

Full title :Hepatitis C methods and protocols / edited by Hengli Tang.

Summary

With the development of robust cell-culturing systems capable of Hepatitis C Virus replication and infection, the past decade has witnessed an explosion of innovative research directed at understanding HCV biology in the context of the authentic replicative cycle of the virus. By providing a compilation of cutting-edge research techniques currently in the use in HCV labs worldwide, Hepatitis C: Methods and Protocols, Second Edition fully updates the previous edition to reflect this progress which promises to unlock the remaining vital information regarding this human pathogen and its interactions. Written in the highly successful Methods in Molecular Biology" series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Fully updated and comprehensives, Hepatitis C: Methods and Protocols, Second Edition serves as a valuable reference guide for basic and clinical researchers using HCV as a model and striving to better understand this critical disease.

Edition Statements

Publication Date:©2009.
Edition:2nd ed

Publication Statements

Publisher(s):Humana Press
Place of Publication:Totowa, N.J
Language:eng
Publication Date:©2009.

Notes

The Physical Object

Description:xxi, 459 pages : : illustrations ; : 24 cm

Edition Identifiers

ISBN:9781588299703, 1588299708, 9781597453943, 1597453943
oclc:ocn297115240
LC Classification:QR201.H46
Dewey Decimal:616.3623 22

Detection and quantification of serum or plasma HCV RNA: Mini review of commercially available assays / H. Le Guillou-Guillemette and F. Lunel-Fabiani -- Simultaneous detection of anti-HCV antibody and HCV core antigen / F. Ansaldi and G. Icardi -- Immunohistochemical detection of HCV proteins in liver tissue / O. Galy [and others] -- Nomenclature and numbering of the hepatitis C virus / C. Kuiken and P. Simmonds -- A real-time taqman method for hepatitis C virus genotyping and methods for further subtyping of isolates / K.J. Rolfe [and others] -- Characterization of HCV quasispecies in the hypervariable region 1 (HVR1) by in vitro translation and mass spectrometry / C. Yea, M. Ayers and R. Tellier -- Purification and crystallization of NS5A domain I of hepatitis C virus / J. Marcotrigiano and T. Tellinghuisen -- NS5A phosphorylation and hyperphosphorylation / P. Neddermann -- Preparation and handling of hepatitis C viral proteins NS3 and NS5B for structural studies / S.D. Marco and A. Carfi -- Structural and functional analysis of the HCV p7 protein / N. Saint [and others] -- HCV replicons: overview and basic protocols / V. Lohmann -- Reverse transcription PCR-based sequence anal…

Subjects

Clinical Laboratory Techniques Hepacivirus Hepacivirus -- Cahiers de laboratoire Hepatitis C virus Hepatitis C virus -- Research Laboratory Manual Laboratory manuals Manuels de laboratoire Médecine -- Laboratoires Medical laboratory technology Techniques et procédures de laboratoire… Virus de l'hépatite C -- Recherche Virus de l'hépatite C.