HIV protocols

by Kalpana, Ganjam V. Prasad, Vinayaka R.

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Title Statements

Full title :HIV protocols / edited by Vinayaka R. Prasad and Ganjam V. Kalpana.

Summary

Despite major advances in the treatment of HIV, there are still many areas requiring additional study. These areas include ways to create efficacious, potent antiretrovirals for suppressing viral loads completely without toxic side effects, the definition of unknown drug targets while fine-tuning known targets, and the ability to better understand the interplay between viral and host factors. In HIV Protocols, Second Edition, expert researchers provide clear, state-of-the-art methods for the study of HIV. Directed toward three specific goals, this text aims to document up-to-date protocols for select aspects of HIV biology, to bring together both virological and immunological approaches in a single, convenient volume, and to present a comprehensive account of a range of techniques not available in any existing HIV protocol book. As a volume in the highly successful Methods in Molecular Biology series, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections containing priceless tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, HIV Protocols, Second Edition is an ideal guide to the wide array of techniques used in fundamental or applied research into the biology and pathogenesis of HIV-1.

Edition Statements

Publication Date:©2009.
Edition:2nd ed

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York, NY
Language:eng
Publication Date:©2009.

The Physical Object

Description:1 online resource (xiv, 457 pages) : : illustrations

Edition Identifiers

ISBN:9781597451703, 1597451703
DOI:10.1007/978-1-59745-170-3
oclc:ocn300493421
LC Classification:QR201.A37
Dewey Decimal:616.079

Methods for viral RNA isolation and PCR amplification for sequencing of near full-length HIV-1 genomes / K.S. Kemal [and others]-Purification of HIV-1 virions by subtilisin digestion or CD45 immunoaffinity depletion for biochemical studies / D.E. Ott-Calculating HIV-1 infectious titre using a virtual TCID50 method / Y. Gao [and others]-Cell-free assays for HIV-1 uncoating / C. Aiken-Real-time pcr analysis of HIV-1 replication post-entry events / J.L. Mbisa [and others]-Analysis of 2-LTR circle junctions of viral DNA in infected cells / D. Mandal and V.R. Prasad-HIV-1 recombination: An experimental assay and a phylogenetic approach / M.D. Moore, M.P. Chin and W.S. Hu-Methods of preparation and analysis of intracellular reverse transcription complexes / A. Fassati-Analysis of viral and cellular proteins in HIV-1 reverse transcription complexes by co-immunoprecipitation / S.N. Iordanskiy and M.I. Bukrinsky-Isolation and analysis of HIV-1 preintegration complexes / A. Engelman-Bisarsenical labeling of HIV-1 for real-time fluorescence microscopy / N.J. Arhel and P. Charneau-Methods for the study of HIV-1 assembly / A.A. Waheed, A. Ono and E.O. Freed-Assembly of immature HIV-1 capsids u…

Subjects

acquired immune deficiency syndrome AIDS (Disease) AIDS (Disease) -- Laboratory manuals Clinical Protocols HIV (Viruses) HIV (Viruses) -- Laboratory manuals HIV Infections HIV infections -- Laboratory manuals HIV Infections -- prevention & control human immunodeficiency viruses Human Pathology Humane pathologie Immunologic Techniques Immunologie Immunology Infections à VIH -- Manuels de laborato… laboratoriummethoden Laboratory Manual Laboratory manuals Laboratory Methods