Introduction to Confocal Fluorescence Microscopy

Introduction to Confocal Fluorescence Microscopy

by Muller, Michiel

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Title Statements

  • Full title :Introduction to Confocal Fluorescence Microscopy / Michiel Müller.

Edition Statements

  • Publication Date:cop. 2006.
  • Edition:2nd ed

Publication Statements

  • Publisher(s):SPIE
  • Place of Publication:Bellingham, Washington
  • Language:eng
  • Publication Date:cop. 2006.

Notes

Symbols and abbreviations Preface 1 Confocal fluorescence microscopy 1.1 The principle 1.2 The theory 1.3 What is the resolution? 1.4 Optical aberrations 2 Implementation 2.1 The microscope objective 2.2 Scanning 2.3 The detection pinhole 2.4 Fluorophores 2.4.1 Absorption and fluorescence 2.4.2 Fluorescent proteins 2.5 The laser 3 Practical limits 4 Digitisation 5 Miscellaneous topics 5.1 PSF Measurement 5.2 Restoration/deconvolution 5.3 The commercial instrument 5.4 Related three-dimensional fluorescence techniques 5.4.1 4? microscopy 5.4.2 Two-photon absorption microscopy A Elements of optical theory A.1 Geometrical optics A.2 Aberration theory A.3 Diffraction B Formulas, relations and definitions C Fourier theory.

The Physical Object

  • Description:xvi, 119 p. : : il. ; : 26 cm.

Edition Identifiers

  • ISBN:9780819460431, 0819460435
  • oclc:ocn954116493

Subjects

Microscopía confocal Microscopía óptica Microscopios de fluorescencia

Writers

Muller, Michiel

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