Laboratory methods in enzymology
by Lorsch, Jon
The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 520 volumes and 40,000 chapters in the collection, much of the material is still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemist…
Read More ↓Title Statements
- Full title :Laboratory methods in enzymology. / edited by Jon Lorsch.
Summary
The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 520 volumes and 40,000 chapters in the collection, much of the material is still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. In this volume, number 545, we have brought.
Read More ↓Edition Statements
- Publication Date:2014.
- Edition:First edition
Publication Statements
- Publisher(s):Elsevier/Academic Press
- Place of Publication:Amsterdam
- Language:eng
- Publication Date:2014.
The Physical Object
- Description:1 online resource (xvii, 272 pages) : : illustrations
Edition Identifiers
- ISBN:9780128003350, 0128003359
- oclc:ocn911266632
- LC Classification:QP601
- Dewey Decimal:572/.70721
Table of Contents
Section 1: Protein protocols/protein precipiation: TCA precipitation -- Section II: Protein protocols/protein pull-down methods: Coimmunoprecipitation of proteins from yeast -- Coupling antibody to cyanogen bromide-activated sepharose -- Analysis of protein-protein interactions by coimmunoprecipitation --Section III: Protein protocols/protein purification: Use and application of hydrophobic interaction chromatography for protein purification -- Hydroxyapatite chromatography: purification strategies for recombinant proteins -- Salting out of proteins using ammonium sulfate precipitation -- Using ion exchange chromatography to purify a recombinantly expressed protein -- Gel filtration chromatography (size exclusion chromatography) of proteins -- Section IV: Protein protocols/purification of membrane proteins: Expression and purification of membrane proteins -- Explantory chapter: choosing the right detergent -- Section V: Protein protocols/SDS PAGE: One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE) -- Coomassie blue staining -- Silver staining of SDS-polyacrylamide gel -- Section VI: Protein protocols/Standard in vitro assays for protein-nucleic acid interactions:…
Read More ↓Subjects
Writers
More from this Series
No reviews yet. Be the first to rate this work.
Related Works
more →
Genetics
Functional performance in older adults
Methods in cell physiology
After extinction
Developmental neuropathology
Files & Downloads
-
Laboratory Methods in Enzymology: Protein Part C …
PDF · 14.5 MB · updated -
Laboratory Methods in Enzymology: Protein Part D…
PDF · 10.5 MB · updated
Rate & Review