Lipid-Protein Interactions: Methods and Protocols

by Kleinschmidt, Jörg H.

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Title Statements

Full title :Lipid-Protein Interactions : Methods and Protocols.

Summary

This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications. Additional protocols were added to examine lipid-protein interactions by mass spectrometry, to use protein microarrays to investigate large sets of various proteins, to study membrane protein dynamics by UV resonance Raman spectroscopy, to analyze peptide-induced pore formation in membranes, and to investigate folding and insertion of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Second Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function.

Edition Statements

Publication Date:2019.
Edition:2nd ed

Publication Statements

Publisher(s):Springer
Place of Publication:New York, NY
Language:eng
Publication Date:2019.

Notes

3.3 Setting Up Experimental Parameters

The Physical Object

Description:1 online resource (685 pages)

Edition Identifiers

ISBN:9781493995127, 149399512X, 9781493995110, 1493995111, 9781493995134, 1493995138, 9781493995141, 1493995146
DOI:10.1007/978-1-4939-9512-7.
oclc:on1122923330
LC Classification:QH301-705
Dewey Decimal:572.57

Intro; Preface; Contents; Contributors; Chapter 1: Multiscale Modeling and Simulation Approaches to Lipid-Protein Interactions; 1 Introduction; 2 Materials; 2.1 Survey of Structural Information; 2.2 Choice of Representation; 2.3 Structure Preparation; 2.4 Membrane Embedding; 2.5 Multiscale Models; 2.6 The Simulation Loop; 2.7 Simulation Protocol; 2.8 Basic Analysis; 2.9 Computational Considerations; 3 Methods; 3.1 Setting Up Membrane Protein and Membrane Peptide Systems; 3.1.1 Inserting a Protein Within a Lipid Bilayer Environment; 3.1.2 Setting Up Peripheral Membrane Protein Systems

3.2 Preparation of Complex Membrane Protein Systems3.2.1 Lamellar and Nonlamellar Starting Structures with CHARMM-GUI; 3.2.2 A Protocol for Simulating Enveloped Virus Particles; 3.3 Setting Up Biologically Realistic Membrane Systems; 3.4 Beyond Membranes: Bacterial Envelopes and Cell Walls; 3.5 Thermodynamics of Lipid-Protein Complex Interactions; 4 Notes; References; Chapter 2: Quartz Crystal Microbalances as Tools for Probing Protein-Membrane Interactions; 1 Introduction; 2 Materials; 2.1 QCM-D System and Accessories; 2.2 Lipid Bilayers; 2.3 Software; 3 Methods; 3.1 Preparations

3.1.1 Cleaning of …