Lipopolysaccharide transport: methods and protocols

by Sperandeo, Paola

Type Book

Rate 0.0 /5

Details

Files

Reviews

Download

Title Statements

Full title :Lipopolysaccharide transport : methods and protocols / edited by Paola Sperandeo.

Summary

This volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Lipopolysaccharide Transport: Methods and Protocols aims to deliver an overview on the multidisciplinary study of LPS transport and to identify new and innovative antibacterial strategies.

Edition Statements

Publication Date:©2022

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:©2022

Notes

Includes index.

The Physical Object

Description:1 online resource (x, 292 pages) : : illustrations (chiefly color).

Edition Identifiers

ISBN:9781071625811, 1071625810
DOI:10.1007/978-1-0716-2581-1
oclc:on1346300425
LC Classification:QP751
Dewey Decimal:612/.01577

Use of Mutagenesis and Functional Screens to Characterize Essential Genes Involved in Lipopolysaccharide Transport -- Generation of Stable and Unmarked Conditional Mutants in Pseudomonas aeruginosa -- Analysing the Function of Essential Genes by Plasmid Shuffling -- Membrane Fractionation by Isopycnic Sucrose Density Gradient Centrifugation for Qualitative Analysis of LPS in Escherichia Coli -- Exploring The Topology of Cytoplasmic Membrane Proteins Involved in Lipopolysaccharide Biosynthesis by in silico and Biochemical Analyses -- Use of Site-Directed Spin Labeling EPR Spectroscopy to Study Protein-LPS Interactions -- A Photo-Crosslinking Approach to Monitor Assembly of an LptD Intermediate with LptE in a Living Cell -- Mass Spectrometry Analysis of Dynamics and Interactions of the LPS translocon LptDE -- Affinity Purification and Co-Immunoprecipitation of Trans-envelope Protein Complexes in Gram-negative Bacteria -- A Bacterial Two-Hybrid System for in vivo assays of Protein-Protein Interactions and Drug Discovery -- Detecting Lipoproteins Sneaking out of the Lipopolysaccharide Leaflet -- Dissecting Lipopolysaccharide Composition and Structure by GC-MS and MALDI Spectrometry --…