Mass Cytometry: Methods and Protocols

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This book details a compilation of up-to-date and cutting-edge protocols in mass cytometry. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on t…

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Title Statements

Full title :Mass Cytometry : Methods and Protocols.

Summary

Edition Statements

Publication Date:2019.

Publication Statements

Publisher(s):Springer
Place of Publication:New York, NY
Language:eng
Publication Date:2019.

Notes

Chapter 6: Titration of Mass Cytometry Reagents

The Physical Object

Description:1 online resource (333 pages)

Edition Identifiers

ISBN:9781493994540, 1493994549
DOI:10.1007/978-1-4939-9454-0.
oclc:on1122911336
LC Classification:QH301-705
Dewey Decimal:571.6

Intro; Preface; Contents; Contributors; Part I: Setting Up a Facility; Chapter 1: Setting Up Mass Cytometry in a Shared Resource Lab Environment; 1 Introduction; 2 Operation Model and Services; 3 Requirements for the Laboratory Setup; 4 Equipment and Materials that Should be in Place; 5 Best Practices in a Mass Cytometry Shared Resource Lab; 5.1 Instrument Quality Control and Performance Tracking; 5.2 Cleaning Procedures and Preventative Maintenance; 5.3 Documentation; 5.4 Project Discussions for Mass Cytometry Experiments; 5.5 Management of Samples and Documentation; 6 Notes; References

Chapter 2: Acquisition, Processing, and Quality Control of Mass Cytometry Data1 Introduction; 1.1 Ion Cloud Formation and TOF Measurement; 1.2 IMD/FCS Generation; 2 Materials; 2.1 Data; 2.2 Software; 2.3 Solutions; 3 Methods; 3.1 Sample Preparation and Acquisition; 3.2 Initial Sample Quality Control (QC); 3.2.1 Bead Intensity; 3.2.2 Coefficient of Variance (CV); 3.2.3 Oxidation; 3.2.4 Signal Stability; 3.3 Normalization; 3.4 Concatenation; 3.5 Randomization; 3.6 Channel Relabeling; 3.7 Doublet Discrimination; 3.8 Debarcoding; 3.9 Live Cell Identification; 4 Notes; References

Part II: Panel Desig…