Mass Spectrometry Handbook

Due to its enormous sensitivity and ease of use, mass spectrometry has grown into the analytical tool of choice in most industries and areas of research. This unique reference provides an extensive library of methods used in mass spectrometry, covering applications of mass spectrometry in fields as diverse as drug discovery, environmental science, forensic science, clinical analysis, polymers, oil composition, doping, cellular research, semiconductor, ceramics, metals and alloys, and homeland security. The book provides the reader with a protocol for the technique described (including sampling.

Cover; WILEY SERIES ON PHARMACEUTICAL SCIENCE AND BIOTECHNOLOGY: PRACTICES, APPLICATIONS AND METHODS; Title page; Copyright page; FOREWORD; PREFACE; CONTRIBUTORS; SECTION I: BIOTECHNOLOGY/PROTEINS; 1 TARGETED PROTEOMICS USING IMMUNOAFFINITY PURIFICATION; 1.1 INTRODUCTION; 1.2 EXPERIMENTAL PROTOCOLS; 1.3 APPLICATIONS OF THE PROTOCOLS; 1.4 CONCLUSION; ACKNOWLEDGMENTS; 2 MASS SPECTROMETRY-BASED METHODS TO INVESTIGATE POSTTRANSLATIONAL PROTEIN MODIFICATIONS BY LIPID PEROXIDATION PRODUCTS; 2.1 PROCEDURE CONTROL.

2.2 IDENTIFICATION OF HNE-MODIFIED PEPTIDES IN BIOLOGICAL SAMPLES BY SOLID-PHASE ENRICHMENT AND NANO-LC-ESI-MS/MS2.3 REAGENTS AND STANDARDS; 2.4 COMMENTARY; ACKNOWLEDGMENTS; 3 IMAGING MASS SPECTROMETRY (IMS) FOR BIOLOGICAL APPLICATION; 3.1 INTRODUCTION; 3.2 APPLICATIONS OF MALDI-IMS; 3.3 EXPERIMENTAL PROCEDURES; 3.4 STATISTICAL PROCEDURES FOR IMS DATA ANALYSIS; 4 METHODOLOGIES FOR IDENTIFYING MICROORGANISMS AND VIRUSES BY MASS CATALOGING OF RNAs; 4.1 INTRODUCTION: THE IMPORTANCE OF MICROBIAL GENOTYPING; 4.2 INFORMATICS: ENABLING ASPECTS OF 16S RRNA AND DATABASE CONSTRUCTION.

4.3 EXPERIMENTAL METHODS: PCR, TRANSCRIPTION, AND ENZYMATIC FRAGMENTATION4.4 DATABASE COMPARISON AND R…