Membrane lipids: methods and protocols
This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel tec…
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- Full title :Membrane lipids : methods and protocols / edited by Charles G. Cranfield.
Summary
This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics.
Read More ↓Edition Statements
- Publication Date:2022
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York, NY
- Language:eng
- Publication Date:2022
The Physical Object
- Description:xi, 300 pages : : illustrations (some color) ; : 26 cm.
Edition Identifiers
- ISBN:9781071618424, 1071618423
- oclc:on1285704387
- LC Classification:QP752.M45
- Dewey Decimal:572.577
Table of Contents
Methods for Forming Giant Unilamellar Fatty Acid Vesicles -- Preparing Ion Channel Switch Membrane-Based Biosensors -- Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely Tethered Lipid Bilayer -- Electrochemical Impedance Spectroscopy as a Convenient Tool to Characterize Tethered Bilayer Membranes -- Measuring Voltage-Current Characteristics of Tethered Bilayer Lipid Membranes to Determine the Electro-Insertion Properties of Analytes -- Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs) -- Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes -- De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer -- Drug Meets Monolayer: Understanding the Interactions of Sterol Drugs with Models of the Lung Surfactant Monolayer Using Molecular Dynamics Simulations -- Establishing a Lipid Bilayer for Molecular Dynamics Simulations -- Initiating Coarse-Grained MD Simulations for Membrane-Bound Proteins -- Small Angle Neutron Scattering of Liposomes: Sample Preparation to Simple Modeling --…
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