Membrane proteins

by Shukla, Arun K.

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Title Statements

Full title :Membrane proteins. / edited by Arun K. Shukla.

Edition Statements

Publication Date:2015.

Publication Statements

Publisher(s):Academic Press
Place of Publication:Waltham, MA
Language:eng
Publication Date:2015.

Notes

VERSEMENT EN LOT.

The Physical Object

Description:1 ressource en ligne.

Edition Identifiers

ISBN:9780128021934, 0128021934
oclc:on1083917178
LC Classification:QP552.M44
Dewey Decimal:572/.696

Front Cover; Membrane Proteins-Engineering, Purification and Crystallization; Copyright; Contents; Contributors; Preface; Section I: Membrane Protein Engineering, Solubilization and Purification; Chapter One: Multicolor Fluorescence-Based Screening Toward Structural Analysis of Multiprotein Membrane Complexes; 1. Introduction; 1.1. Screening approaches for membrane proteins and membrane protein complexes; 1.2. Multicolor fluorescence-based screening of multiprotein membrane complexes

1.3. The major histocompatibility complex class I peptide-loading complex: A challenge for structural biology of membrane...2. Production of Fluorescently Labeled TAP Complexes in P. pastoris; 2.1. Construct design; 2.2. Transformation; 2.3. Colony screening; 2.4. Large-scale expression; 2.5. Orthogonal purification; 3. Production of Fluorescently Labeled TAP Complexes in Mammalian Cells; 3.1. Construct design; 3.2. Transfection; 3.3. Orthogonal purification; 4. Multicolor Fluorescence-Based Screening Approaches; 4.1. MC-FSEC analysis; 4.2. In-gel fluorescence and immunoblotting

4.3. Monitoring peptide binding to TAP4.4. Measurement of MHC I surface presentation by flow cytometry; 5. Summary; Acknow…