Methods in cell biology

by Pfister, K. Kevin

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Title Statements

Full title :Methods in cell biology.

Edition Statements

Publication Date:©2016
Edition:First edition

Publication Statements

Publisher(s):Academic Press
Place of Publication:Amsterdam, [Netherlands]
Language:eng
Publication Date:©2016

Notes

Description based upon print version of record.

The Physical Object

Description:1 online resource (552 p.).

Edition Identifiers

ISBN:0128033541, 9780128033548, 0128033444, 9780128033449
oclc:on1113542701
LC Classification:QP363
Dewey Decimal:599.0188

Front Cover; Series Editors; The Neuronal Cytoskeleton, Motor Proteins, and Organelle Trafficking in the Axon; Copyright; Contents; Contributors; Preface; 1 -- Axonal transport: The orderly motion of axonal structures; 1. PULSE-LABELING STUDIES OF AXONAL TRANSPORT; 2. LIVE-CELL IMAGING OF AXONAL TRANSPORT; 2.1 FC and the Movement of Vesicular Cargoes; 2.2 Slow Axonal Transport and the Movement of Cytoskeletal Polymers; 2.3 Neurofilaments are Transported in Axons; 2.4 Microtubules and Slow Axonal Transport; 2.5 SCb and the Movement of Soluble Proteins of Axoplasm; 3. SUMMARY; REFERENCES

2 -- Live-cell imaging of neurofilament transport in cultured neuronsINTRODUCTION; 1. CULTURING NEURONS; 1.1 General Considerations; 1.2 Culture Media; 1.2.1 Media for superior cervical ganglion neuron cultures; 1.2.2 Media for cortical neuron cultures; 1.2.3 Media for dorsal root ganglion neuron cultures; 1.3 Coverslips and Culture Dishes; 1.3.1 Glass-bottomed dishes; 1.3.2 Sterilizing and coating coverslips and glass-bottomed dishes; 1.4 Superior Cervical Ganglion Neuron Cultures; 1.5 Cortical Neuron Cultures; 1.5.1 Preparation of coverslips for glial sandwich cultures

1.5.2 Preparation of a sus…