Methods in cilia & flagella

Methods in cilia & flagella

by Basto, Renata

Type Book
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Title Statements

  • Full title :Methods in cilia & flagella / edited by Renata Basto and Wallace F. Marshall ; contributors, Zachary D. Abbott [and eighty-six others].

Edition Statements

  • Publication Date:©2015

Publication Statements

  • Publisher(s):Academic Press
  • Place of Publication:Amsterdam, [Netherlands]
  • Language:eng
  • Publication Date:©2015

Notes

Description based upon print version of record.

2.2 Immunostaining of Dissociated Cardiac Cells

The Physical Object

  • Description:1 online resource (616 p.).

Edition Identifiers

  • ISBN:0128026405, 9780128026403
  • oclc:on1113178031
  • LC Classification:QP215
  • Dewey Decimal:571.67

Table of Contents

Front Cover; Methods in Cell Biology; Series Editors; Methods in Cell BiologyMethods in Cilia & FlagellaVolume 127Edited byRenata BastoInstitut Curie, FranceWallace F. MarshallD ...; Contents; Contributors; Preface; 1. Imaging centrosomes and cilia in the mouse kidney; Introduction; 1. Methods; 1.1 Isolation of Kidneys from Adult Mouse; 1.2 Isolation of Kidneys from Mouse Embryo; 1.3 Fixation and Preparation of Slides; 1.4 Antibody Staining of Fixed Samples; 1.4.1 Antigen retrieval; 1.4.2 Antibody staining; 1.4.3 Fluorescence microscopy; 1.5 Ex vivo Culture of Embryonic Kidney; Conclusion

2.2.1 Materials2.2.2 Method; 2.2.3 Analysis; 3. Localization of PCP Signaling Pathway Proteins in Multiciliated Airway Epithelial Cells; 3.1 Observing Vang-Like 1 and Frizzled 6 Localization in the In vivo Multiciliated Airway Epithelium; 3.1.1 Materials; 3.1.2 Method; 3.1.3 Analysis; 3.2 Observing Vang-Like 1 and Frizzled 6 Localization in In vitro Differentiated Multiciliated Airway Epithelial Cells; 3.2.1 Materials; 3.2.2 Method; 3.2.3 Analysis; Conclusions; Acknowledgments; References; 4. Imaging and analyzing primary cilia in cardiac cells; Introduction

1. Imaging Cilia In situ, in Fixed H…

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