Title Statements
- Full title :Methods in cilia & flagella / edited by Renata Basto and Wallace F. Marshall ; contributors, Zachary D. Abbott [and eighty-six others].
Edition Statements
- Publication Date:©2015
Publication Statements
- Publisher(s):Academic Press
- Place of Publication:Amsterdam, [Netherlands]
- Language:eng
- Publication Date:©2015
Notes
Description based upon print version of record.
2.2 Immunostaining of Dissociated Cardiac Cells
The Physical Object
- Description:1 online resource (616 p.).
Edition Identifiers
- ISBN:0128026405, 9780128026403
- oclc:on1113178031
- LC Classification:QP215
- Dewey Decimal:571.67
Table of Contents
Front Cover; Methods in Cell Biology; Series Editors; Methods in Cell BiologyMethods in Cilia & FlagellaVolume 127Edited byRenata BastoInstitut Curie, FranceWallace F. MarshallD ...; Contents; Contributors; Preface; 1. Imaging centrosomes and cilia in the mouse kidney; Introduction; 1. Methods; 1.1 Isolation of Kidneys from Adult Mouse; 1.2 Isolation of Kidneys from Mouse Embryo; 1.3 Fixation and Preparation of Slides; 1.4 Antibody Staining of Fixed Samples; 1.4.1 Antigen retrieval; 1.4.2 Antibody staining; 1.4.3 Fluorescence microscopy; 1.5 Ex vivo Culture of Embryonic Kidney; Conclusion
2.2.1 Materials2.2.2 Method; 2.2.3 Analysis; 3. Localization of PCP Signaling Pathway Proteins in Multiciliated Airway Epithelial Cells; 3.1 Observing Vang-Like 1 and Frizzled 6 Localization in the In vivo Multiciliated Airway Epithelium; 3.1.1 Materials; 3.1.2 Method; 3.1.3 Analysis; 3.2 Observing Vang-Like 1 and Frizzled 6 Localization in In vitro Differentiated Multiciliated Airway Epithelial Cells; 3.2.1 Materials; 3.2.2 Method; 3.2.3 Analysis; Conclusions; Acknowledgments; References; 4. Imaging and analyzing primary cilia in cardiac cells; Introduction
1. Imaging Cilia In situ, in Fixed H…
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