Methods in Enzymology

by Kelman, Zvi

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Title Statements

Full title :Methods in Enzymology. / edited by Zvi Kelman ; contributors, Luke W. Arbogast [and fifty others].

Edition Statements

Publication Date:©2016

Publication Statements

Publisher(s):Academic Press
Place of Publication:Amsterdam, [Netherlands]
Language:eng
Publication Date:©2016

Notes

Description based upon print version of record.

2.3.2. Sample Conditioning for NMR Spectroscopy

The Physical Object

Description:1 online resource (513 p.).

Edition Identifiers

ISBN:0128030747, 9780128030745, 0128030496, 9780128030493
oclc:on1113727722
LC Classification:RS425
Dewey Decimal:615.3

Front Cover; Isotope Labeling of Biomolecules -- Applications; Copyright; Contents; Contributors; Preface; Section I: Nuclear Magnetic Resonance Spectroscopy; Chapter One: Application of Natural Isotopic Abundance 1H-13C- and 1H-15N-Correlated Two-Dimensional NMR for Evaluation o ...; 1. Introduction; 2. Preparation of Protein Therapeutic Samples; 2.1. Considerations for Sample Conditions; 2.2. Sample Preparation of the Intact NISTmAb; 2.3. Enzymatic Digest of mAbs into Fab and Fc Domains; 2.4. Sample Preparation of Filgrastim; 3. Acquisition of 2D Spectra; 3.1. Amide 1H-15N-Correlated Spectra

3.2. Methyl 1H-13C-Correlated Spectra3.3. Nonuniform Sampling Method; 4. Processing 2D-Correlated Spectra; 4.1. Processing Uniformly Sampled Data; 4.2. Processing Nonuniformly Sampled Data; 5. Comparative Analysis of 2D-Correlated Spectra; 6. Perspectives; Acknowledgments; References; Chapter Two: A Semiautomated Assignment Protocol for Methyl Group Side Chains in Large Proteins; 1. Introduction; 2. Labeling of Side Chain Methyl Groups for Large Proteins; 3. Methyl Labeling Protocol for the cAMP-Dependent Protein Kinase A; 3.1. Materials; 3.2. Purification of PKA-C; 3.3. Materials

4. Semia…