Mycobacteria protocols

by Parish, Tanya Stoker, Neil G.

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Title Statements

Full title :Mycobacteria protocols / edited by Tanya Parish and Neil G. Stoker.

Summary

Laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease, including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis.

Edition Statements

Publication Date:©1998.

Publication Statements

Publisher(s):Humana Press
Place of Publication:Totowa, N.J
Language:eng
Publication Date:©1998.

The Physical Object

Description:xiv, 472 pages : : illustrations ; : 24 cm.

Edition Identifiers

ISBN:0896034712, 9780896034716
oclc:ocm39170391
LC Classification:QR82.M8
Dewey Decimal:579.3/74

Mycobacteria : bugs and bugbears / Tanya Parish, Neil G. Stoker -- Mycobacteria : general culture methodology and safety considerations / Bryan W. Allen -- Isolation of genomic DNA from mycobacteria / John T. Belisle, Michael G. Sonnenberg -- Nucleic-acid extraction : plasmid/cosmid DNA / Bonnie B. Plikaytis, C. Harold King -- Pulsed field gel electrophoresis for mycobacteria / Wolfgang J. Philipp, Stephen Gordon, Amalio Telenti, Stewart T. Cole -- Extraction of RNA from mycobacteria / Eshwar Mahenthiralingam -- Preparation of cell-free extracts from mycobacteria / Tanya Parish, Paul R. Wheeler -- Preparation of cell-wall fractions from mycobacteria / Gurdyal S. Besra -- Using MycDB on the World-Wide Web / Staffan Bergh, Stewart T. Cole -- Conjugating DNA into mycobacteria / Julie-Ann Gavigan, Carlos Martín -- Electroporation of mycobacteria / Tanya Parish, Neil G. Stoker -- Mycobacteriophages / Gary J. Sarkis, Graham F. Hatfull -- Chemical mutagenesis of mycobacteria / Lucy A. Brooks -- Tn611 transposon mutagenesis in Mycobacterium smegmatis using a temperature-sensitive delivery system / Esther Pérez, Julie-Ann Gavigan, Isabel Otal, Christophe Guilhot, Vladimir Pelicic, Brigitte…

Subjects

ADN -- Physiologie Bacterial Genetics Bacterial genetics -- Laboratory manuals Biologie moléculaire -- méthodes DNA DNA -- Physiology Génétique bactérienne Génétique bactérienne -- Manuels de lab… Laboratory Manual Laboratory manuals Manuels de laboratoire Molecular Biology -- methods Mycobacteria Mycobacteria -- Laboratory manuals Mycobactériacées -- Manuels de laborato… Mycobacterium Mycobacterium -- croissance et développ… Mycobacterium -- genetics Mycobacterium -- Génétique Mycobacterium -- growth & development