Mycobacteria protocols
by Parish, Tanya Stoker, Neil G.
In Mycobacteria Protocols, laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease-including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. The…
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- Full title :Mycobacteria protocols / edited by Tanya Parish and Neil G. Stoker.
Summary
In Mycobacteria Protocols, laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease-including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis. Mycobacteria Protocols contains a unique collection of both established and recently developed techniques that have been optimized and adapted for use with mycobacteria. Offering an eminently practical introduction for all those carrying out basic or applied research in this important class of bacteria, Mycobacteria Protocols is certain to become the new standard reference in the field, one from which even expert researchers will learn many time-saving techniques.
Read More ↓Edition Statements
- Publication Date:©1998.
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:Totowa, N.J
- Language:eng
- Publication Date:©1998.
The Physical Object
- Description:1 online resource (xiv, 472 pages) : : illustrations
Edition Identifiers
- ISBN:0896034712, 9780896034716, 9781592595761, 1592595766, 128083689X, 9781280836893, 9786610836895, 6610836892
- DOI:10.1385/0896034712.
- oclc:ocm54986776
- LC Classification:QR82.M8
- Dewey Decimal:579.3/74
Table of Contents
Mycobacteria : bugs and bugbears / Tanya Parish, Neil G. Stoker -- Mycobacteria : general culture methodology and safety considerations / Bryan W. Allen -- Isolation of genomic DNA from mycobacteria / John T. Belisle, Michael G. Sonnenberg -- Nucleic-acid extraction : plasmid/cosmid DNA / Bonnie B. Plikaytis, C. Harold King -- Pulsed field gel electrophoresis for mycobacteria / Wolfgang J. Philipp, Stephen Gordon, Amalio Telenti, Stewart T. Cole -- Extraction of RNA from mycobacteria / Eshwar Mahenthiralingam -- Preparation of cell-free extracts from mycobacteria / Tanya Parish, Paul R. Wheeler -- Preparation of cell-wall fractions from mycobacteria / Gurdyal S. Besra -- Using MycDB on the World-Wide Web / Staffan Bergh, Stewart T. Cole -- Conjugating DNA into mycobacteria / Julie-Ann Gavigan, Carlos Martín -- Electroporation of mycobacteria / Tanya Parish, Neil G. Stoker -- Mycobacteriophages / Gary J. Sarkis, Graham F. Hatfull -- Chemical mutagenesis of mycobacteria / Lucy A. Brooks -- Tn611 transposon mutagenesis in Mycobacterium smegmatis using a temperature-sensitive delivery system / Esther Pérez, Julie-Ann Gavigan, Isabel Otal, Christophe Guilhot, Vladimir Pelicic, Brigitte…
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