PCR GURU: an ultimate benchtop reference for molecular biologists
Title Statements
- Full title :PCR GURU : an ultimate benchtop reference for molecular biologists / Ayaz Najafov, Gerta Hoxhaj.
Edition Statements
- Publication Date:©2017
Publication Statements
- Publisher(s):Elsevier, Academic Press is an imprint of Elsevier
- Place of Publication:Amsterdam
- Language:eng
- Publication Date:©2017
The Physical Object
- Description:xv, 89 pages : : color illustrations ; : 23 cm
Edition Identifiers
- ISBN:9780128042311, 0128042311
- oclc:ocn980378209
- LC Classification:QP606.D46
- Dewey Decimal:572/.43
Table of Contents
Chapter 1. Introduction 1.1 -- A bit of history; 1.2 -- What is PCR?; 1.3 -- PCR components; 1.4 -- Exponential nature of PCR; 1.5 -- Popularity of PCR -- Chapter 2. -- Procedure; 2.1 -- Primer design; 2.2 -- Standard PCR setup; 2.3 -- Controls; 2.4 -- Cycling parameters; 2.4.1 Denaturation; 2.4.2 Annealing; 2.4.3 Extension -- Chapter 3. Good PCR Practices -- Chapter 4. Optimization and Troubleshooting; 4.1 -- Optimization rules -- General optimization strategies -- 4.3. -- Troubleshooting specific problems -- Chapter 5. Tips and Tricks -- Chapter 6. Special Cases -- Glossary and Abbreviations -- Appendix A -- Agarose Gel Electrophoresis Protocol -- Appendix B -- Silver Staining of DNA Gels -- Appendix C -- Plasmid Miniprep Protocol (by using "QIAprep Spin Miniprep" Kit) -- Appendix D -- Restriction Enzyme Digestion Protocol -- Appendix E -- Protocol for DNA Concentration Estimation Using Hoechst 33258 -- Appendix F -- Formulas -- Appendix G -- Common Primers for Sequencing, Promoters, Housekeeping Genes, and Reporters -- Appendix H -- Tags -- Appendix I -- Thermostable DNA polymerases -- Appendix J -- Example of a Daily PCR Log -- Appendix K -- Common Buffers and Solutions -- App…
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