Title Statements
- Full title :PCR guru : an ultimate benchtop reference for molecular biologists / Ayaz Najafov, Harvard Medical School, Department of Cell Biology, Boston, Massachusetts, Gerta Hoxhaj, Harvard T.H. Chan School of Public Health, Department of Genetics and Complex Diseases, Boston, Massachusetts.
Edition Statements
- Publication Date:2017.
Publication Statements
- Publisher(s):Academic Press is an imprint of Elsevier
- Place of Publication:London, United Kingdom
- Language:eng
- Publication Date:2017.
The Physical Object
- Description:xv, 89 pages : : illustrations, charts ; : 23 cm.
Edition Identifiers
- ISBN:0128042311, 9780128042311
- oclc:on1001514258
- Dewey Decimal:572.33
Table of Contents
1.Introduction -- 1.1.A Bit of History -- 1.2.What is PCR? -- 1.3.PCR Components -- 1.4.Exponential Nature of PCR -- 1.5.Popularity of PCR -- 2.Procedure -- 2.1.Primer Design -- 2.2.Standard PCR Setup -- 2.3.Controls -- 2.4.Cycling Parameters -- 3.Good PCR Practices -- 4.Optimization and Troubleshooting -- 4.1.Optimization Rules -- 4.2.General Optimization Strategies -- 4.3.Troubleshooting Specific Problems -- 5.Tips and Tricks -- 6.Special Cases.
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