Plant cell division: methods and protocols

by Caillaud, Marie-Cécile

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Title Statements

Full title :Plant cell division : methods and protocols / edited by Marie-Cécile Caillaud.

Summary

This new edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Cell Division: Methods and Protocols, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression.

Edition Statements

Publication Date:2022
Edition:Second edition

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:2022

The Physical Object

Description:1 online resource (1 PDF file (xi, 267 pages)) : : illustrations (chiefly color).

Edition Identifiers

ISBN:9781071617441, 1071617443
DOI:10.1007/978-1-0716-1744-1
oclc:on1283736120
LC Classification:QK725
Dewey Decimal:571.8/44

Methods to Visualize the Actin Cytoskeleton during Plant Cell Division -- Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana -- A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips -- Assaying Cyclin-Dependent Kinase Activity in Synchronized Algal Cultures -- Analysis of Commitment Point Attainment in Algae Dividing by Multiple Fission -- Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical Stage Confocal Microscopy -- Transient Overexpression of E2Fb to Induce Cell Divisions in Nicotiana benthamiana Pavement Cells -- Kinematic Growth Analysis of Rice (Oryza sativa) Leaf -- Studying Cell Division Plane Positioning in Early-Stage Embryos -- Means to Quantify Vascular Cell File Numbers in Different Tissues -- Confocal Analysis of Arabidopsis Root Cell Divisions in 3D: A Focus on the Endodermis -- Quantification of Cell Division Angles in the Arabidopsis Root -- A Quantitative Method for Evaluating Phragmoplast Morphology -- Analyses of Protein Turnover at the Cell Plate by Fluorescence Recovery After Photobleaching during Cytokinesis -- Cell Cycle Synchronization and Time-Lapse Imaging of Cytokinetic …

Subjects

Botanique Botany Cell Culture Techniques Cell division Cell division -- Laboratory manuals Cellules -- Manuels de laboratoire. -- … Laboratory Manual Laboratory manuals Manuels de laboratoire Plant Cells Plant cells and tissues Plant cells and tissues -- Laboratory m… Plantes -- Manuels de laboratoire. -- C…