Plant cytogenetics

by Singh, Ram J.

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Title Statements

Full title :Plant cytogenetics / Ram J. Singh.

Edition Statements

Publication Date:2016.
Edition:Third edition

Publication Statements

Publisher(s):CRC Press
Place of Publication:Boca Raton
Language:eng
Publication Date:2016.

Notes

Previous edition: 2003.

The Physical Object

Description:1 online resource : : illustrations (black and white)

Edition Identifiers

ISBN:9781439884195, 1439884196, 9781315356990, 1315356996, 9781315337937, 1315337932, 9781315374611, 1315374617
oclc:ocn964918996
LC Classification:QK981.35
Dewey Decimal:572.82

Cover -- Half Title -- Title Page -- Copyright Page -- Dedication -- Table of Contents -- Preface -- Acknowledgments -- Author -- Chapter 1: Introduction -- Chapter 2: Handling of Plant Chromosome -- 2.1 Introduction -- 2.2 Squash Technique: Mitotic and Meiotic Chromosomes -- 2.2.1 Collection of Roots -- 2.2.2 Pretreatment of Roots -- 2.2.2.1 Ice-Cold Water -- 2.2.2.2 8-Hydroxyquinoline -- 2.2.2.3 Colchicine -- 2.2.2.4 Ü-Bromonaphthalene -- 2.2.2.5 Para-Dichlorobenzene -- 2.2.3 Fixation -- 2.2.3.1 Carnoy's Solution 1 -- 2.2.3.2 Carnoy's Solution 2 -- 2.2.3.3 Propionic Acid Alcohol Solution -- 2.2.4 Staining of Chromosomes -- 2.2.4.1 Aceto-Carmine Staining -- 2.2.4.2 Feulgen Staining -- 2.2.4.3 Alcoholic-Hydrochloric Acid-Carmine -- 2.2.4.4 Lacto-Propionic-Orcein -- 2.2.4.5 Carbol Fuchsin Staining -- 2.2.4.6 Giemsa Staining -- 2.3 Smear Technique for Plant Chromosomes -- 2.3.1 Chromosome Preparation from Roots -- 2.3.2 Chromosome Preparation from Cell Suspension and Callus -- 2.3.2.1 Suspension Culture of Celery (Apium graveolens) -- 2.3.2.2 Callus Culture of Brassica carinata -- 2.3.3 Chromosome Preparation from Flowers -- 2.3.4 Chromosome Preparation from Shoots -- 2.4 Pollen Sta…