Plant Long Non-Coding RNAs: Methods and Protocols

by Chekanova, Julia A.

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Title Statements

Full title :Plant Long Non-Coding RNAs : Methods and Protocols.

Summary

This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions. The chapters in this book cover how to catalog lncRNAs in various plant species; determining subcellular localization; protein interactions; structures; and RNA modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and innovative, Plant Long Non-Coding RNAs: Methods and Protocols is a valuable resource that aids researchers in understanding the functions of lncRNAs in different plant species, and helps them explore currently uncharted facets of plant biology.

Edition Statements

Publication Date:2019.

Publication Statements

Publisher(s):Springer
Place of Publication:New York, NY
Language:eng
Publication Date:2019.

Notes

1 Introduction

The Physical Object

Description:1 online resource (473 pages)

Edition Identifiers

ISBN:9781493990450, 1493990454
DOI:10.1007/978-1-4939-9045-0.
oclc:on1122913765
LC Classification:QH301-705
Dewey Decimal:580

Intro; Dedication; Preface; Contents; Contributors; Chapter 1: An Overview of Methodologies in Studying lncRNAs in the High-Throughput Era: When Acronyms ATTACK!; 1 Introduction; 2 Identification of lncRNAs Using High-Throughput Methodologies; 2.1 Hybridization-Based Approaches; 2.2 Sequence Tag-Based Approaches; 2.2.1 Serial Analysis of Gene Expression (SAGE); 2.2.2 Massively Parallel Signature Sequencing (MPSS); 2.2.3 Cap Analysis of Gene Expression (CAGE); 2.3 RNA Sequencing (RNA-seq)

2.4 Parallel Analysis of RNA Ends (PARE)/Genome-Wide Mapping of Uncapped and Cleaved Transcripts (GMUCT)/Degradome-Seq2.5 Transcript Isoform Sequencing (TIF-seq); 2.6 Nascent RNA Sequencing; 2.6.1 Global Run-On Sequencing (GRO-Seq); 2.6.2 Precision Nuclear Run-On Sequencing (PRO-Seq); 2.6.3 Native Elongating Transcript Sequencing (NET-Seq); 2.6.4 BRIC-Seq/BrU-Seq/BrUChase-Seq; 3 Analyzing the Biological and Molecular Functions of lncRNAs; 3.1 Tissue or Cell Type-Specific Analysis; 3.1.1 Laser Microdissection (LM); 3.1.2 Fluorescence-Activated Cell Sorting (FACS)

3.1.3 Isolation of Nuclei Tagged in Specific Cell Types (INTACT)3.2 In Situ Hybridization (ISH) and Fluorescence In Situ Hybridization …