Plant Metabolomics: Methods and Protocols
This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field. The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioi…
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- Full title :Plant Metabolomics : Methods and Protocols.
Summary
This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field. The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.
Read More ↓Edition Statements
- Publication Date:May 2018
Publication Statements
- Publisher(s):Humana Press, Springer [distributor]
- Place of Publication:New York
- Language:eng
- Publication Date:May 2018
The Physical Object
- Description:1 online resource (xiv, 355 p. :) : ill.
Edition Identifiers
- ISBN:9781493978182, 1493978187
- oclc:on1152999739
- LC Classification:QK1-989
- Dewey Decimal:580
Table of Contents
The Importance of Experimental Design, Quality Assurance, and Control in Plant Metabolomics Experiments -- Standard Key Steps in Mass Spectrometry-Based Plant Metabolomics Experiments: Instrument Performance and Analytical Method Validation -- Guidelines for Sample Normalization to Minimize Batch Variation for Large-Scale Metabolic Profiling of Plant Natural Genetic Variance -- Gas Chromatography-Mass Spectrometry-Based 13C-Labeling Studies in Plant Metabolomics -- Carbon Atomic Survey for Identification of Selected Metabolic Fluxes -- Quantification of Low-Abundant Phosphorylated Carbohydrates Using HILIC-QqQ-MS/MS -- Quantification of Soluble Sugars and Sugar Alcohols by LC-MS/MS -- Untargeted Analysis of Semi-Polar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation -- Extraction of Plant Lipids for LC-MS-Based Untargeted Plant Lipidomics -- Semi-Targeted Lipidomics of Plant Acyl Lipids Using UPLC-HR-MS in Combination with a Data-Independent Acquisition Mode -- Plant Lipidomics Using UPLC-QTOF-MS -- Targeted LC-MS Analysis for Plant Secondary Metabolites -- An UHPLC-MS/MS Method for Target Profiling of Stress-Re…
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