Plant nitric oxide: methods and protocols

by Gupta, Kapuganti Jagadis

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Title Statements

Full title :Plant nitric oxide : methods and protocols / edited by Kapuganti Jagadis Gupta, National Institute of Plant Genome Research, New Delhi, India

Summary

With numerous methods available for the quantification of nitric oxide (NO), this detailed book explores their advantages and disadvantages in order to provide a foundation for further research in plant nitric oxide. After providing a useful practical guide to choosing a technique for measuring NO from plant materials, the book continues with chapters on chemiluminescence, diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory methods, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Nitric Oxide: Methods and Protocols serves as a valuable guide to all researchers working and intending to work in the field of plant nitric oxide research

Edition Statements

Publication Date:2016

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:2016

The Physical Object

Description:xi, 203 Seiten : Illustrationen

Edition Identifiers

ISBN:9781493935987, 1493935984, 9781493936007, 149393600X
oclc:ocn949877855
LC Classification:QK898.N58
Dewey Decimal:572.542

Overview of methods in plant nitric oxide (NO) research : why do we always need to use multiple methods? / Hideo Yamasaki [und weitere] -- Chemiluminescence detection of nitric oxide from roots, leaves, and root mitochondria / Aakanksha Wany [und weitere] -- Nitric oxide measurement from purified enzymes and estimation of scavenging activity by gas Phase chemiluminescence method / Aprajita Kumari [und weitere] -- Localization of nitric oxide in wheat roots by DAF fluorescence / Aakanksha Wany and Kapuganti Jagadis Gupta -- Nitric oxide (NO) measurements in stomatal guard cells / Srinivas Agurla, Gunja Gayatri, and Agepati S. Raghavendra -- Methods to detect nitric oxide in plants : are DAFs really measuring NO? / Stefan Ruemer [und weitere] -- Novel protocol for detection of nitric oxide in plants / Prachi Jain, Anisha David, and Satish C. Bhatla -- Detection of nitric oxide by electron paramagnetic resonance spectroscopy : spin-trapping with iron-dithiocarbamates / Luisa B. Maia and José J.G. Moura -- Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR) / Andrea Galatro and Susana Puntarulo -- Laser-based methods for detection o…