Protein chromatography: methods and protocols
by Loughran, Sinéad T. Walls, Dermot
This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as…
Read More ↓Title Statements
- Full title :Protein chromatography : methods and protocols / edited by Dermot Walls, Sinéad T. Loughran
Summary
This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography
Read More ↓Edition Statements
- Publication Date:2016
- Edition:Second edition
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York
- Language:eng
- Publication Date:2016
The Physical Object
- Description:x, 426 Seiten : Illustrationen : 25.4 x 17.8 cm, 0 g
Edition Identifiers
- ISBN:1493964100, 9781493964109, 9781493964123, 1493964127
- DOI:10.1007/978-1-4939-6412-3
- oclc:ocn962026958
- LC Classification:QP551
- Dewey Decimal:572/.6
Table of Contents
Synopsis of proteins and their purification / Dermot Walls, Gary Cooney, and Sinéad T. Loughran -- Gel-filtration chromatography / Ciarán Ó'Fágáin, Philip M. Cummins, and Brendan F. O'Connor -- Immunoaffinity chromatography : concepts and applications / Jenny Fitzgerald [und weitere] -- Avoiding poteolysis during protein purification / Barry J. Ryan and Gary T. Henehan -- Scale-up of protein purification : downstream processing issues / John Joseph Milne -- Phage display : a powerful technology for the generation of high-specificity affinity reagents from alternative immune sources / William J.J. Finlay [und weitere] -- Protein stability : enhancement and measurement / Ciarán Ó'Fágáin -- Tagging recombinant proteins to enhance solubility and aid purification / Sinéad T. Loughran and Dermot Walls -- Storage and lyophilization of pure proteins / Ciarán Ó'Fágáin and Keith Colliton -- Differential precipitation and solubilization of proteins / Barry J. Ryan and Gemma K. Kinsella -- Ion-exchange chromatography : basic principles and application / Philip M. Cummins, Keith D. Rochfort, and Brendan F. O'Connor -- Protein quantitation and analysis of purity / Eva M. Campion, Sinéad T. Loug…
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