Proteoglycans: methods and protocols

by Rédini, Françoise

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Title Statements

Full title :Proteoglycans : methods and protocols / edited by Francoise Redini.

Summary

Now recognized as a reservoir for growth factors and cytokines modulating cell activation status and turnover, proteoglycans have stimulated great amount of interest and research. In Proteoglycans: Methods and Protocols, experts in the field examine issues of basic concepts and up-to-date analysis methods for proteoglycan and glycosaminoglycan (GAG) at the protein and saccharide levels. The volume continues with the multifunctional aspect of proteoglycans, highlighted through three relevant examples of proteoglycans with highly different strucutures: serglycin, aggrecan, and heparin sulphate proteoglycans. The final chapter describes proteoglycan involvement in the pathogenesis of various disorders and their potential therapeutic value in osteo-articular diseases. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Proteoglycans: Methods and Protocols is an ideal guide for scientists attempting to pursue further research in this vital and complex area of study

Edition Statements

Publication Date:©2012.

Publication Statements

Publisher(s):Humana Press, Springer
Place of Publication:New York
Language:eng
Publication Date:©2012.

The Physical Object

Description:xiv, 359 pages : : illustrations (some color) ; : 25 cm

Edition Identifiers

ISBN:9781617794971, 161779497X, 9781617794988, 1617794988
oclc:ocn752068529
LC Classification:QP552.P73
Dewey Decimal:572.68

Proteoglycans : gene cloning -- Proteoglycan : site mapping and site-directed mutagenesis -- Mapping of the wnt/beta-catenin/TCF response elements in the human versican promoter -- Gene silencing in mouse embryonic stem cells -- A novel strategy for a splice-variant selective gene ablation : the example of the versican v0/v2 knockout -- Detection of neurocan in cerebrospinal fluid -- Glycosaminoglycan chain analysis and characterization (glycosylation/epimerization) -- Characterization of glycosaminoglycans by tandem vibrational microspectroscopy and multivariate data analysis -- Glycosaminoglycans : oligosaccharide analysis by liquid chromatography, capillary electrophoresis, and specific labeling -- Brain chondroitin/dermatan sulfate, from cerebral tissue to fine structure : extraction, preparation, and fully automated chip-electrospray mass spectrometric analysis -- Use of neutrons reveals the dynamics of cell surface glycosaminoglycans -- Following protein-glycosaminoglycan polysaccharide interactions with differential scanning fluorimetry -- In vivo scintigraphic imaging of proteoglycans -- Serglycin : the master of the mast cell -- Analysis of aggrecan catabolism by immunobl…

Subjects

Aufsatzsammlung Laboratory Manual Laboratory manuals Labortechnik Manuels de laboratoire Protéoglycanes -- Manuels de laboratoire Proteoglycans Proteoglycans -- analysis Proteoglycans -- metabolism Proteoglycans -- physiology Proteoglycans--Laboratory manuals Proteoglykane