Quantitative Imaging in Cell Biology

Quantitative Imaging in Cell Biology: Methods in Cell Biology

by Waters, Jennifer

Type Book
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This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detec…

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Title Statements

  • Full title :Quantitative Imaging in Cell Biology : Methods in Cell Biology.

Summary

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols fo.

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Edition Statements

  • Publication Date:2014.

Publication Statements

  • Publisher(s):Elsevier Science
  • Place of Publication:Burlington
  • Language:eng
  • Publication Date:2014.

Notes

5.5. Other Fluorescent Probes.

The Physical Object

  • Description:1 online resource (609 pages).

Edition Identifiers

  • ISBN:9780124202016, 0124202012
  • oclc:ocn882769798
  • LC Classification:QH505.A1 .W3 2014
  • Dewey Decimal:574.0153

Table of Contents

Front Cover; Quantitative Imaging in Cell Biology; Copyright; Contents; Contributors; Preface; Chapter 1: Concepts in quantitative fluorescence microscopy; 1.1. Accurate and Precise Quantitation; 1.2. Signal, Background, and Noise; 1.3. Optical Resolution: The Point Spread Function; 1.4. Choice of Imaging Modality; 1.5. Sampling: Spatial and Temporal; 1.5.1. 2D sampling; 1.5.2. 3D sampling; 1.5.3. Temporal sampling; 1.6. Postacquisition Corrections; 1.6.1. Background subtraction; 1.6.2. Flat-field correction; 1.6.3. Photobleaching; 1.6.4. Storing and processing images for quantitation.

1.7. Making Compromises1.8. Communicating Your Results; Acknowledgment; References; Chapter 2: Practical considerations of objective lenses for application in cell biology; Introduction; 2.1. Optical Aberrations; 2.1.1. On-axis aberrations; 2.1.2. Off-axis aberrations; 2.2. Types of Objective Lenses; 2.2.1. Optical corrections; 2.2.2. Numerical aperture; 2.3. Objective Lens Nomenclature; 2.4. Optical Transmission and Image Intensity; 2.5. Coverslips, Immersion Media, and Induced Aberration; 2.5.1. Optical path length; 2.5.2. Correction collars; 2.5.3. Cover glass; 2.5.4. Immersion media.

2.6. Cons…

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Subjects

Cell Biology Cytologie Cytology Imagerie en biologie Imaging systems in biology Microscopie microscopy

Writers

Waters, Jennifer Wittmann, Torsten

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