Quantitative thin-layer chromatography

Quantitative thin-layer chromatography: a practical survey

by Spangenberg, Bernd

Type Book
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Thin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometri…

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Title Statements

  • Full title :Quantitative thin-layer chromatography : a practical survey / Bernd Spangenberg, Colin F. Poole, Christel Weins.

Summary

Thin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometric data evaluation and validation. An important feature is the coverage of effect-directed biological.

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Edition Statements

  • Publication Date:©2011.

Publication Statements

  • Publisher(s):Springer
  • Place of Publication:Heidelberg
  • Language:eng
  • Publication Date:©2011.

The Physical Object

  • Description:1 online resource (xv, 388 pages) : : illustrations (some color)

Edition Identifiers

  • ISBN:9783642107290, 364210729X, 3642107273, 9783642107276
  • DOI:10.1007/978-3-642-10729-0
  • oclc:ocn701369149
  • LC Classification:QD79.C8
  • Dewey Decimal:543.84

Table of Contents

History of Planar Chromatography -- The Theoretical Basis of Thin-Layer Chromatography (TLC) -- The Stationary Phase in Thin Layer Chromatography (TLC) -- The Mobile Phase in Adsorption and Partition Chromatography -- Preparing and Applying Samples -- Basis for TLC Development Techniques -- Specific Staining Reactions -- Bioeffective-linked Analysis in Modern HPTLC -- Planar Chromatography Detectors -- Diffuse Reflectance from TLC Layers -- Fluorescence in TLC Layers -- Chemometrics in HPTLC -- Reciprocal Model -- Statistics for Quantitative TLC -- Planning an Analysis and Validation in TLC.

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