Reverse chemical genetics: methods and protocols
Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established met…
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- Full title :Reverse chemical genetics : methods and protocols / edited by Hisashi Koga.
Summary
Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study.
Read More ↓Edition Statements
- Publication Date:©2009.
Publication Statements
- Publisher(s):Humana Press
- Place of Publication:New York, NY
- Language:eng
- Publication Date:©2009.
The Physical Object
- Description:1 online resource (xi, 272 pages) : : illustrations (some color)
Edition Identifiers
- ISBN:9781607612322, 1607612321
- DOI:10.1007/978-1-60761-232-2
- oclc:ocn436089328
- LC Classification:QH430
- Dewey Decimal:572.8
Table of Contents
ORFeome cloning / Osamu Ohara -- Systematic cloning of an ORFeome using the gateway system / Akihisa Matsuyama and Minoru Yoshida -- High-throughput construction of ORF clones for production of the recombinant proteins / Hisashi Yamakawa -- Computational overview of GPCR gene universe to support reverse chemical genomics study / Makiko Suwa and Yukiteru Ono -- High-performance gene expression module analysis tool and its application to chemical toxicity data / Wataru Fujibuchi [and others] -- Human protein reference database and human proteinpedia as discovery tools for systems biology / T.S. Keshava Prasad, Kumaran Kandasamy, and Akhilesh Pandey -- High-throughput production of the recombinant proteins expressed in Escherichia coli utilizing cDNA resources / Kiyo Shimada and Hisashi Koga -- An insect cell-free system for recombinant protein expresion using cDNA resources / Takashi Syuzuki [and others] -- Recombinant protein production by a kaiko-baculovirus system / Hidekazu Nagaya -- Pulse-chase experiment for the analysis of protein stability in cultured mammalian cells by covalent fluorescent labeling of fusion proteins / Kei Yamaguchi [and others] -- Application of 2D-DIGE in…
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