Sample preparation in LC-MS bioanalysis

1.4.3.2 Common SPE Platforms in LC-MS Bioanalysis1.4.3.3 General SPE Workflows; 1.4.3.4 Other Formats of SPE; 1.4.4 Combination of PPT, LLE, and/or SPE in LC-MS Bioanalysis; 1.4.4.1 Combination of PPT and LLE; 1.4.4.2 Combination of PPT and SPE; 1.4.4.3 Combination of LLE and SPE; 1.4.5 Summary; References; Chapter 2 Online Extraction and Column Switching Techniques in LC-MS Bioanalysis; 2.1 Introduction; 2.2 System Configuration; 2.2.1 Single-column System; 2.2.2 Dual-column System; 2.2.3 Staggered Parallel Online Extraction Systems; 2.3 Commonly Used Online Extraction Techniques.

1.3.2 Nonspecific Binding1.3.3 Protein Binding; 1.3.4 Blood-to-plasma Ratio and Red Blood Cell Partition; 1.4 Most Commonly Used Sample Preparation Methods in LC-MS Bioanalysis; 1.4.1 Protein Precipitation (PPT); 1.4.1.1 Water-miscible Organic Solvents; 1.4.1.2 Acids; 1.4.2 Liquid-Liquid Extraction (LLE); 1.4.2.1 Mechanism of LLE and Extraction Recovery; 1.4.2.2 Solvent in LLE; 1.4.2.3 General Procedures in LLE; 1.4.2.4 Application of LLE in LC-MS Bioanalysis; 1.4.2.5 Other Formats of LLE; 1.4.3 Solid-phase Extraction (SPE); 1.4.3.1 SPE Stationary Phases (Sorbents).

2.3.1 Turbulent/High Flow Chromatography2.3.2 Restricted Access Media; 2.3.3 Monolithic Materials; 2.3.4 Hydrophilic Interaction Liquid Chromatography; 2.3.5 Immunoaffinity Extraction; 2.3.6 Disposable Extraction Cartridges -- Online SPE; 2.3.7 Online Extraction of Dried Blood Spot (DBS) Samples; 2.3.8 SPE-MS; 2.4 Considerations for Utilizing Online Extraction Techniques; 2.4.1 Advantages and Limitations; 2.4.2 Strategies for Online Extraction Method Development; 2.5 Summary; References; Chapter 3 Equilibrium Dialysis, Ultracentrifugation, and Ultrafiltration in LC-MS Bioanalysis.

3.1 Introduction3.2 Challenges and Considerations; 3.3 Experimental Procedures; 3.3.1 Equilibrium Dialysis; 3.3.2 Ultrafiltration; 3.3.3 Ultracentrifugation; 3.4 Summary; References; Chapter 4 Phospholipid Depletion Techniques in LC-MS Bioanalysis; 4.1 Introduction; 4.2 Impact of Phospholipids on Bioanalytical Methods; 4.3 Investigating Matrix Effects Associated with Phospholipids; 4.4 Minimizing Matrix Effects Associated with Phospholipids; 4.4.1 Sample Dilution; 4.4.2 Column Manipulations; 4.4.3 Internal Standards; 4.4.4 Ionization Choice; 4.4.5 Other Experimental Modifications.

Intro; Title Page; Copyright Page; Contents; List of Contributors; Preface; List of Abbreviations; Part I Current Sample Preparation Techniques in LC-MS Bioanalysis; Chapter 1 Basic Sample Preparation Techniques in LC-MS Bioanalysis: Protein Precipitation, Liquid-Liquid Extraction, and Solid-Phase Extraction; 1.1 Introduction; 1.2 Physicochemical Properties of Drugs and Their Metabolites; 1.2.1 Hydrophilicity vs. Lipophilicity of Analyte(s); 1.2.2 Protolytic Properties of Analyte(s); 1.3 Pre-analytical Variables of Analyte(s) of Interest in Biological Matrix; 1.3.1 Stability.

Includes bibliographical references and index.