Separating Cells

Separating Cells: The basics provides user-friendly and practical guidance to the techniques most commonly used to separate cells. The book offers a concise overview of the fundamental principles and explains the 'what, how and why'. This title will be of considerable interest to newcomers to these techniques.

6.2 The purification of viable spermatozoa from bovine semen.

Book Cover; Half-Title; Title; Copyright; Contents; Abbreviations; Preface; Glossary; Chapter 1 Preparation of cell suspensions; 1. Diversity of cells; 2. Why separate living cells?; 3. Preparation of suspensions of single cells; 3.1 Isolation of cells from suspension; 3.2 Isolation of cells from solid tissues; Animal and human tissue; Other tissue; 4. Viability measurements; 5. Separation of viable and nonviable cells; 6. Characterization of cells; 6.1 Light microscopy; 6.2 Phase-contrast microscopy; 6.3 Electron microscopy; 6.4 Confocal microscopy; 6.5 Flow cytometry; 6.6 Magnetic beads.

6.7 ImmunohistochemistryCell preparation; Fixatives; Antibodies; Mounting media; Detection method; 6.8 Metabolic characteristics; Further reading; References; Protocol 1.1 Isolation of cells from bronchial alveolar lavage; Protocol 1.2 Isolation of cells from peritoneal lavage; Protocol 1.3 Isolation of cells from soft tissue by mechanical disruption; Protocol 1.4 Preparation of vascular smooth muscle cells by mechanical disruption and outgrowth in culture; Protocol 1.5 Preparation of endothelial cells from omental fat by mechanical disruption and enzymatic digestion.

Protocol 1.6 Isolation of…