Single cell methods: sequencing and proteomics

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"This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reag…

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Title Statements

Full title :Single cell methods : sequencing and proteomics / edited by Valentina Proserpio.

Summary

Edition Statements

Publication Date:2019

Publication Statements

Publisher(s):Humana Press
Place of Publication:New York
Language:eng
Publication Date:2019

The Physical Object

Description:xvi, 459 pages : : illustrations (black and white, and colour) ; : 26 cm.

Edition Identifiers

ISBN:9781493992393, 1493992392
oclc:on1083031072

Lab setup and tissue preparation. Setting up a single-cell genomic laboratory -- Tissue handling and dissociation for single-cell RNA-seq -- Single cell trancriptomic analysis. Full-length single-cell RNA sequencing with Smart-seq2 -- CEL-Seq2: single-cell RNA sequencing by multiplexed linear amplification -- Single-cell RNA-seq by multiple annealing and tailing-based quantitative single-cell RNA-seq (MATQ-seq) -- Single-Cell RNA sequencing with Drop-Seq -- Chromium 10× single-cell 3′ mRNA sequencing of tumor-infiltrating lymphocytes -- Seq-Well: a sample-efficient, portable picowell platform for massively parallel single-cell RNA sequencing -- Single-cell tagged reverse transcription (STRT-seq) -- Single-cell RNA-sequencing of peripheral blood mononuclear cells with ddSEQ -- High-throughput single-cell real-time quantitative PCR analysis -- Single-cell dosing and mRNA sequencing of suspension and adherent cells using the Polaris system -- Targeted TCR amplification from single-cell cDNA libraries -- Single cell genomic and epigenomic analysis. Sequencing the genomes of single cells -- Studying DNA methylation in single-cell format with scBS-seq -- Single-cell 5fC sequencing -- Si…

Subjects

Cytological Techniques Cytologie -- Manuels de laboratoire Cytologie -- Technique Cytology Cytology -- Laboratory manuals Cytology -- Technique Laboratory Manual Laboratory manuals Manuels de laboratoire Single-Cell Analysis -- methods