Single Cell Methods: Sequencing and Proteomics

by Proserpio, Valentina

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Title Statements

Full title :Single Cell Methods : Sequencing and Proteomics.

Summary

This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab.

Edition Statements

Publication Date:2019.

Publication Statements

Publisher(s):Springer
Place of Publication:New York, NY
Language:eng
Publication Date:2019.

Notes

3.1.3 Digestion of Primers (See Note 3)

The Physical Object

Description:1 online resource (452 pages)

Edition Identifiers

ISBN:9781493992409, 1493992406, 9781493992393, 1493992392, 9781493992416, 1493992414, 9781493992423, 1493992422
DOI:10.1007/978-1-4939-9240-9
oclc:on1122916062
LC Classification:QH301-705
Dewey Decimal:571.6

Intro; Preface; Acknowledgment; Contents; Contributors; Part I: Lab Setup and Tissue Preparation; Chapter 1: Setting Up a Single-Cell Genomic Laboratory; 1 Introduction; 2 Materials; 2.1 Experimental Procedure and QC; 2.2 Workplace, Equipment, Reagents and Samples Handling; References; Chapter 2: Tissue Handling and Dissociation for Single-Cell RNA-Seq; 1 Introduction; 2 Materials; 2.1 Human Blood; 2.2 Human Spleen; 2.3 Human Lymph Node; 2.4 Human Lung; 2.5 Human Skin; 2.6 Red Blood Cell Lysis; 2.7 Dead Cell Removal; 2.8 Immune Cell Enrichment; 2.9 Cell Counting; 3 Methods; 3.1 Blood

3.1.1 Tissue Collection and Preparation3.1.2 Neutrophils and Mononuclear Fraction Separation; 3.1.3 RBC Lysis; 3.1.4 Dead Cell Removal; 3.2 Human Spleen; 3.2.1 Tissue Collection and Preparation; 3.2.2 Mechanical Dissociation; 3.2.3 RBC Lysis; 3.2.4 Dead Cell Removal; 3.3 Human Lymph Node; 3.3.1 Tissue Collection and Preparation; 3.3.2 Mechanical Dissociation and Enzymatic Digestion; 3.3.3 Dead Cell Removal; 3.4 Human Lung; 3.4.1 Tissue Collection and Preparation; 3.4.2 Mechanical Dissociation and Enzymatic Digestion; 3.4.3 RBC Lysis; 3.4.4 Dead Cell Removal; 3.4.5 Immune Cell Enrichment

3.5 Human Sk…